Glyk, A.; Solle, D.; Scheper, T.; Beutel, S.: Optimization of PEG-salt aqueous two-phase systems by design of experiments. In: Chemometrics and Intelligent Laboratory Systems 149 (2015), S. 12-21. DOI: https://doi.org/10.1016/j.chemolab.2015.09.014
Zusammenfassung: | |
Since the mechanism governing the partitioning behavior of biomolecules, such as proteins and enzymes, in polyethylene glycol (PEG)-salt aqueous two-phase systems (ATPS) is complex and not easily predictable, many laborious experiments have to be performed for an optimization of these systems, causing increased overall cost. However, the multivariate statistical design of experiments (DoE) methodology is representing a promising and efficient optimization technique which can overcome the limitations of traditional optimization methods. Therefore, DoE has emerged as a powerful and efficient optimization tool for PEG-salt ATPS, since it is faster, more efficient and cost-effective, allowing a simultaneous and rigorous evaluation of process/system parameters. In the present review, different DoE process steps are represented to highlight the feasibility of this approach to operate as a promising and efficient optimization tool, thus facilitating the evaluation of the partitioning behavior, recovery and purification of different proteins and enzymes in PEG-salt ATPS. In this context, several experimental designs, such as factorial and response surface designs, have been discussed and evaluated by statistical regression analysis and analysis of variance (ANOVA), as well as various applications of PEG-salt ATPS using DoE have been outlined which may further promote the optimization of these systems. | |
Lizenzbestimmungen: | CC BY-NC-ND 4.0 Unported |
Publikationstyp: | Article |
Publikationsstatus: | acceptedVersion |
Erstveröffentlichung: | 2015 |
Die Publikation erscheint in Sammlung(en): | Naturwissenschaftliche Fakultät |
Pos. | Land | Downloads | ||
---|---|---|---|---|
Anzahl | Proz. | |||
1 | Germany | 199 | 32,10% | |
2 | China | 92 | 14,84% | |
3 | United States | 83 | 13,39% | |
4 | Mexico | 28 | 4,52% | |
5 | India | 28 | 4,52% | |
6 | Malaysia | 26 | 4,19% | |
7 | Thailand | 14 | 2,26% | |
8 | United Kingdom | 13 | 2,10% | |
9 | Iran, Islamic Republic of | 12 | 1,94% | |
10 | Brazil | 12 | 1,94% | |
andere | 113 | 18,23% |
Hinweis
Zur Erhebung der Downloadstatistiken kommen entsprechend dem „COUNTER Code of Practice for e-Resources“ international anerkannte Regeln und Normen zur Anwendung. COUNTER ist eine internationale Non-Profit-Organisation, in der Bibliotheksverbände, Datenbankanbieter und Verlage gemeinsam an Standards zur Erhebung, Speicherung und Verarbeitung von Nutzungsdaten elektronischer Ressourcen arbeiten, welche so Objektivität und Vergleichbarkeit gewährleisten sollen. Es werden hierbei ausschließlich Zugriffe auf die entsprechenden Volltexte ausgewertet, keine Aufrufe der Website an sich.