Transit peptide elements mediate selective protein targeting to two different types of chloroplasts in the single-cell C4 species Bienertia sinuspersici

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Wimmer, Diana; Bohnhorst, Philipp; Shekhar, Vinay; Hwang, Inhwan; Offermann, Sascha: Transit peptide elements mediate selective protein targeting to two different types of chloroplasts in the single-cell C4 species Bienertia sinuspersici. In: Scientific Reports 7 (2017), 41187. DOI:

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Sum total of downloads: 146

Bienertia sinuspersici is a terrestrial plant that performs C4 photosynthesis within individual cells through operating a carbon concentrating mechanism between different subcellular domains including two types of chloroplasts. It is currently unknown how differentiation of two highly specialized chloroplasts within the same cell occurs as no similar cases have been reported. Here we show that this differentiation in photosynthetic cells of B. sinuspersici is enabled by a transit peptide (TP) mediated selective protein targeting mechanism. Mutations in the TPs cause loss of selectivity but not general loss of chloroplast import, indicating the mechanism operates by specifically blocking protein accumulation in one chloroplast type. Hybrid studies indicate that this selectivity is transferable to transit peptides of plants which perform C4 by cooperative function of chloroplasts between two photosynthetic cells. Codon swap experiments as well as introducing an artificial bait mRNA show that RNA affects are not crucial for the sorting process. In summary, our analysis shows how the mechanism of subcellular targeting to form two types of chloroplast within the same cell can be achieved. This information is not only crucial for understanding single-cell C4 photosynthesis; it provides new insights in control of subcellular protein targeting in cell biology.
License of this version: CC BY 4.0 Unported
Document Type: Article
Publishing status: publishedVersion
Issue Date: 2017
Appears in Collections:Naturwissenschaftliche Fakultät

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pos. country downloads
total perc.
1 image of flag of Germany Germany 110 75.34%
2 image of flag of United States United States 16 10.96%
3 image of flag of China China 5 3.42%
4 image of flag of Japan Japan 3 2.05%
5 image of flag of Latvia Latvia 2 1.37%
6 image of flag of Czech Republic Czech Republic 2 1.37%
7 image of flag of Switzerland Switzerland 2 1.37%
8 image of flag of Estonia Estonia 1 0.68%
9 image of flag of Brazil Brazil 1 0.68%
10 image of flag of Australia Australia 1 0.68%
    other countries 3 2.05%

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