The Tibetan Plateau (TP) comprises sensitive alpine environments such as grassland biomes. Climatic changes and intensifying land use threaten these ecosystems. Therefore, it is important to understand the response of ecosystems to changing biotic and abiotic factors. The translocation of dissolved organic matter from glacial and terrestrial to aqueous systems is an important aspect of this response, specifically when characterizing changing conditions of freshwater resources and sensitive limnic ecosystems on the TP. Via changes in its chemical composition, characteristics, transformation and processing of DOM can be tracked. Three catchments of the Nam Co watershed of the TP (Niyaqu, Qugaqie and Zhagu) and the lake were investigated to understand how site specific terrestrial processes and seasonality affect the composition of DOM and alteration of organic compounds in streams and the lake of this endorheic basin. Four hypotheses were tested: H1 The natural diversity in the Nam Co watershed controls site specific effects on DOM composition. H2 Seasonal effects on DOM composition are driven by warm and moist summers influenced from the Indian summer monsoon (ISM) and cold and dry winters. H3/ H4a Site specific effects on DOM diminish by means of biological decomposition and photooxidation of DOM during the stream path / in the lake. Alongside H4b organic matter of the Nam Co Lake is independent from catchment influences, given by an autochthonous source of DOM.A multi-parameter approach was applied, consitsing of water chemistry parameters (pH, electric conductivity, cations and anions, dissolved inorganic carbon), concentration of dissolved organic carbon (DOC), DOM characteristics (chromophoric DOM, fluorescence DOM and δ13C of DOM) and DOM ultra-high resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Sampling was conducted for three seasons, freshet in 2018, the phase of the ISM in 2019 and post-ISM baseflow in 2019. Alongside a watershed-wide plant cover estimate was composed, to explore the link between differences in DOM characteristics and degree of green plant cover. Sampling covers stream water, as well as endmember samples such as: glacial effluents, water of springs and water from an alpine wetland. The lake was covered by sampling the brackish zone and the lake pelagial and the lake surface.The composition of DOM differed between the three endmember groups and between stream samples of catchments. Glaciers showed a dual DOM source, indicating a glacial microbiome and compounds derived from burned fossil fuels. Springs differed based on their geographic location. Upland waters showed limited inputs of alpine pastures: lowland springs displayed influences of yak faeces with microbial reworked DOM, indicated by less negative δ13C and nitrogen. Wetlands were distinguished by more eutrophic conditions by highest concentrations in DOC and high amounts in N-heteroatoms. Streams were site specific with input sources derived from glaciers, wetlands, groundwater, intense animal husbandry and a plant-derived phenolic signature from alpine pastures aligned to the degree of plant cover. Seasonality affected DOM characteristics in stream water. During freshet, DOM was plant-derived, as was during baseflow conditions. A flush of dissolved organic carbon, accompanied by a compositional shift towards more microbial derived DOM was observed during the ISM season.Processing of DOM in streams was limited to the biolabile fraction of DOM of the glacial biome. Transformation of DOM was overruled by the constant input of plant derived phenolic DOM compounds from alpine pastures. Consequentially, the brackish intermixing zone showed the inflow of terrestrial DOM into the lake. In contrast, lake water exhibited distinct DOM characteristics, by lowest amounts in aromatic molecular compounds and DOM rich in 13C. This suggested intense processing of phenolic, terrestrial derived DOM by photooxidation, as well as a seasonally stable autochthonous DOM source derived from algae and microorganisms in lake water. In conclusion, DOM characteristics are largely influenced by local endmembers such as glaciers, springs and wetlands. Seasonality shows that shifts in the onset, and changes in the intensity of the ISM can largely modify DOM composition. Processing of DOM took place mainly in the lake. The study revealed that DOM is suited to function as a monitoring agent in this lake watershed. Hence, DOM is a helpful tool to understand changes in ecosystems, and forthcoming, to safeguard sensitive ecosystems of the TP.