Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites

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Höfler, S.; Lukat, P.; Blankenfeldt, W.; Carlomagno, T.: Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites. In: Scientific reports 11 (2021), 17561. DOI:

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Sum total of downloads: 88

Box C/D ribonucleoprotein complexes are RNA-guided methyltransferases that methylate the ribose 2’-OH of RNA. The central ‘guide RNA’ has box C and D motifs at its ends, which are crucial for activity. Archaeal guide RNAs have a second box C’/D’ motif pair that is also essential for function. This second motif is poorly conserved in eukaryotes and its function is uncertain. Conflicting literature data report that eukaryotic box C’/D’ motifs do or do not bind proteins specialized to recognize box C/D-motifs and are or are not important for function. Despite this uncertainty, the architecture of eukaryotic 2’-O-methylation enzymes is thought to be similar to that of their archaeal counterpart. Here, we use biochemistry, X-ray crystallography and mutant analysis to demonstrate the absence of functional box C’/D’ motifs in more than 80% of yeast guide RNAs. We conclude that eukaryotic Box C/D RNPs have two non-symmetric protein assembly sites and that their three-dimensional architecture differs from that of archaeal 2’-O-methylation enzymes.
License of this version: CC BY 4.0 Unported
Document Type: Article
Publishing status: publishedVersion
Issue Date: 2021
Appears in Collections:Naturwissenschaftliche Fakultät

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pos. country downloads
total perc.
1 image of flag of Germany Germany 37 42.05%
2 image of flag of United States United States 21 23.86%
3 image of flag of Russian Federation Russian Federation 9 10.23%
4 image of flag of Czech Republic Czech Republic 9 10.23%
5 image of flag of China China 4 4.55%
6 image of flag of Kazakhstan Kazakhstan 1 1.14%
7 image of flag of Japan Japan 1 1.14%
8 image of flag of Iran, Islamic Republic of Iran, Islamic Republic of 1 1.14%
9 image of flag of India India 1 1.14%
10 image of flag of Indonesia Indonesia 1 1.14%
    other countries 3 3.41%

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