Apart from innovators and early adopters, industrial manufacturers are still looking for adequate guidancefor the orientation, prioritization, and development of Industrie 4.0 initiatives. Case studies of best practiceshave proven beneficial for the identification of use cases in manufacturing companies. Therefore, the aim ofthis paper is to develop an orientation guide for Industrie 4.0 with best practice use cases structured intothematic categories of production system areas. The orientation guide gathers relevant use cases for theadaption in industrial implementations. As a first practical application, the guide will be rolled out in aninternational study to primarily support regional manufacturing companies from Germany in thedevelopment of own Industrie 4.0 use cases. However, the guide is universally applicable to anymanufacturing company and provides useful insights for the implementation of Industrie 4.0 use casesglobally.