Meyer, K.V.; Siller, I.G.; Schellenberg, J.; Gonzalez Salcedo, A.; Solle, D. et al.: Monitoring cell productivity for the production of recombinant proteins by flow cytometry : An effective application using the cold capture assay. In: Engineering in Life Sciences 21 (2021), Nr. 5, S. 288-293. DOI: https://doi.org/10.1002/elsc.202000049
Zusammenfassung: | |
Due to the increasing economic and social relevance of biotherapeutics, their production processes are continually being reconsidered and reoptimized in an effort to secure higher product concentrations and qualities. Monitoring the productivity of cultured cells is therefore a critically important part of the cultivation process. Traditionally, this is achieved by determining the overall product titer by high performance liquid chromatography (HPLC), and then calculating the specific cell productivity based on this titer and an associated viable cell density. Unfortunately, this process is typically time‐consuming and laborious. In this study, the productivity of Chinese Hamster Ovary (CHO) cells expressing a monoclonal antibody was analyzed over the course of the cultivation process. In addition to calculating the specific cell productivity based on the traditional product titer determined by HPLC analysis, culture productivity of single cells was also analyzed via flow cytometry using a cold capture assay. The cold capture assay is a cell surface labelling technique described by Brezinsky et al., which allows for the visualization of a product on the surface of the producing cell. The cell productivity results obtained via HPLC and the results of cold capture assay remained in great accordance over the whole cultivation process. Accordingly, our study demonstrates that the cold capture assay offers an interesting, comparatively time‐effective, and potentially cheaper alternative for monitoring the productivity of a cell culture. | |
Lizenzbestimmungen: | CC BY 4.0 Unported |
Publikationstyp: | Article |
Publikationsstatus: | publishedVersion |
Erstveröffentlichung: | 2021 |
Die Publikation erscheint in Sammlung(en): | Naturwissenschaftliche Fakultät |
Pos. | Land | Downloads | ||
---|---|---|---|---|
Anzahl | Proz. | |||
1 | Germany | 93 | 51,96% | |
2 | United States | 32 | 17,88% | |
3 | Taiwan | 9 | 5,03% | |
4 | Russian Federation | 6 | 3,35% | |
5 | Korea, Republic of | 6 | 3,35% | |
6 | China | 6 | 3,35% | |
7 | Ireland | 5 | 2,79% | |
8 | United Kingdom | 4 | 2,23% | |
9 | Denmark | 4 | 2,23% | |
10 | Hong Kong | 2 | 1,12% | |
andere | 12 | 6,70% |
Hinweis
Zur Erhebung der Downloadstatistiken kommen entsprechend dem „COUNTER Code of Practice for e-Resources“ international anerkannte Regeln und Normen zur Anwendung. COUNTER ist eine internationale Non-Profit-Organisation, in der Bibliotheksverbände, Datenbankanbieter und Verlage gemeinsam an Standards zur Erhebung, Speicherung und Verarbeitung von Nutzungsdaten elektronischer Ressourcen arbeiten, welche so Objektivität und Vergleichbarkeit gewährleisten sollen. Es werden hierbei ausschließlich Zugriffe auf die entsprechenden Volltexte ausgewertet, keine Aufrufe der Website an sich.