Viheriälä, T.; Sorvari, J.; Ihalainen, T.O.; Mörö, A.; Grönroos, P. et al.: Culture surface protein coatings affect the barrier properties and calcium signalling of hESC-RPE. In: Scientific Reports 11 (2021), Nr. 1, 933. DOI: https://doi.org/10.1038/s41598-020-79638-8
Zusammenfassung: | |
Human pluripotent stem cell-derived retinal pigment epithelium (RPE) transplantation is currently under evaluation as treatment for macular degeneration. For therapeutic applications, cryostorage during cell production is typically needed with potential consequences to cell functionality. We have previously shown that the culture substrate affects human embryonic stem cell-derived RPE (hESC-RPE) properties in fresh cultures. Here, we aimed to further identify the role of RPE basement membrane proteins type IV collagen (Col-IV), laminin (LN), and nidogen-1 in the maturation and functionality of hESC-RPE after cryopreservation. In addition to cell attachment and morphology, transepithelial electrical resistance, expression of key RPE proteins, phagocytosis capacity and Ca2+ signalling were analysed. After cryostorage, attachment of hESC-RPE on culture surfaces coated with Col-IV alone was poor. Combining Col-IV and LN with or without nidogen-1 significantly improved cell attachment and barrier properties of the epithelium. Furthermore, functional homogeneity of the hESC-RPE monolayer was enhanced in the presence of nidogen-1. Our results suggest that the choice of coating proteins for the cell culture may have implications to the functional properties of these cells after cryostorage cell banking. © 2021, The Author(s). | |
Lizenzbestimmungen: | CC BY 4.0 Unported |
Publikationstyp: | Article |
Publikationsstatus: | publishedVersion |
Erstveröffentlichung: | 2021 |
Die Publikation erscheint in Sammlung(en): | Fakultät für Maschinenbau |
Pos. | Land | Downloads | ||
---|---|---|---|---|
Anzahl | Proz. | |||
1 | Germany | 16 | 36,36% | |
2 | United States | 12 | 27,27% | |
3 | China | 5 | 11,36% | |
4 | Argentina | 3 | 6,82% | |
5 | Vietnam | 2 | 4,55% | |
6 | No geo information available | 2 | 4,55% | |
7 | Taiwan | 1 | 2,27% | |
8 | Russian Federation | 1 | 2,27% | |
9 | Indonesia | 1 | 2,27% | |
10 | Switzerland | 1 | 2,27% |
Hinweis
Zur Erhebung der Downloadstatistiken kommen entsprechend dem „COUNTER Code of Practice for e-Resources“ international anerkannte Regeln und Normen zur Anwendung. COUNTER ist eine internationale Non-Profit-Organisation, in der Bibliotheksverbände, Datenbankanbieter und Verlage gemeinsam an Standards zur Erhebung, Speicherung und Verarbeitung von Nutzungsdaten elektronischer Ressourcen arbeiten, welche so Objektivität und Vergleichbarkeit gewährleisten sollen. Es werden hierbei ausschließlich Zugriffe auf die entsprechenden Volltexte ausgewertet, keine Aufrufe der Website an sich.