Hydrolysis of chlorogenic acid in apple juice using a p‐coumaryl esterase of Rhizoctonia solani

Download statistics - Document (COUNTER):

Siebert, Mareike; Berger, Ralf Günter; Pfeiffer, Franziska: Hydrolysis of chlorogenic acid in apple juice using a p‐coumaryl esterase of Rhizoctonia solani. In: Journal of the Science of Food and Agriculture 99 (2019) Nr. 14, S. 6644-6648. DOI: https://doi.org/10.1002/jsfa.9940

Repository version

To cite the version in the repository, please use this identifier: https://doi.org/10.15488/10344

Selected time period:


Sum total of downloads: 93

BACKGROUND: Apple juice is rich in polyphenolic compounds, especially in chlorogenic acid. A sour and bitter taste has been attributed to the compound. Chlorogenic acid in coffee powder was quickly hydrolysed by a p‐coumaryl esterase of Rhizoctonia solani (RspCAE) at its optimal pH of 6.0. It was unknown, however, if RspCAE would also degrade chlorogenic acid under the strongly acidic conditions (pH 3.3) present in apple juice.RESULTS: Treatment of apple juice with RspCAE led to a chlorogenic acid degradation from 53.38 ± 0.94 mg L−1 to 21.02 ± 1.47 mg L−1. Simultaneously, the caffeic acid content increased from 6.72 ± 0.69 mg L−1 to 19.33 ± 1.86 mg/L−1. The aroma profile of the enzymatically treated sample and a control sample differed in only one volatile. Vitispirane had a higher flavour dilution factor in the treated juice. Sensory analysis showed no significant difference in the taste profile ( p < 0.05).CONCLUSION: These results demonstrated a high stability and substrate specificity of RspCAE. An increase in caffeic acid and a concurrent decrease in chlorogenic acid concentration may exert a beneficial effect on human health.
License of this version: CC BY-NC 4.0 Unported
Document Type: Article
Publishing status: publishedVersion
Issue Date: 2019
Appears in Collections:Naturwissenschaftliche Fakultät

distribution of downloads over the selected time period:

downloads by country:

pos. country downloads
total perc.
1 image of flag of Germany Germany 52 55.91%
2 image of flag of United States United States 14 15.05%
3 image of flag of China China 14 15.05%
4 image of flag of No geo information available No geo information available 2 2.15%
5 image of flag of Russian Federation Russian Federation 1 1.08%
6 image of flag of Portugal Portugal 1 1.08%
7 image of flag of Norway Norway 1 1.08%
8 image of flag of Korea, Republic of Korea, Republic of 1 1.08%
9 image of flag of Hong Kong Hong Kong 1 1.08%
10 image of flag of Switzerland Switzerland 1 1.08%
    other countries 5 5.38%

Further download figures and rankings:


Zur Erhebung der Downloadstatistiken kommen entsprechend dem „COUNTER Code of Practice for e-Resources“ international anerkannte Regeln und Normen zur Anwendung. COUNTER ist eine internationale Non-Profit-Organisation, in der Bibliotheksverbände, Datenbankanbieter und Verlage gemeinsam an Standards zur Erhebung, Speicherung und Verarbeitung von Nutzungsdaten elektronischer Ressourcen arbeiten, welche so Objektivität und Vergleichbarkeit gewährleisten sollen. Es werden hierbei ausschließlich Zugriffe auf die entsprechenden Volltexte ausgewertet, keine Aufrufe der Website an sich.

Search the repository