As several countries have committed themselves to the promotion of inclusive school systems, teachers might feel overwhelmed by the additional competencies needed for inclusive teaching. Beyond an increase in specialised knowledge, these competencies include a coherent belief system to facilitate the adoption of inclusive practices. Currently, there is scarce knowledge concerning the foundation of teachers’ beliefs and values and the possible connections between these personality traits and inclusive practices. Based on the theory of cognitive hierarchy, we investigated the predictive ability of the value of universalism in shaping sentiments, attitudes and concerns about inclusive education (RQ1), as well as their links to the anticipated enjoyment of teaching in inclusive settings as an indicator of enthusiasm for teaching (RQ2). Within a sample of 229 biology pre-service teachers (Mage = 22.9 years, SDage = 3.5 years; 76% female, 68% bachelor) we found universalism to be a direct predictor of sentiments, attitudes and concerns regarding inclusive education. Furthermore, universalism was the strongest predictor of anticipated enjoyment of teaching in inclusive settings, while only sentiments about inclusive education were not predictive for enjoyment. The study illustrates how deeper underlying values like universalism is connected to beliefs about inclusive education and subsequent motivations in the classroom. When teacher educators intend to motivate pre-service teachers to teach in inclusive settings, these variables should be kept in mind, though further study must be done on the generalisability of the results for pre-service teachers of other school subjects. © 2019 The Authors. Journal of Research in Special Educational Needs published by John Wiley & Sons Ltd on behalf of National Association for Special Educational Needs