Taidi, B.; Lebernede, G.; Koch, L.; Perre, P.; Chichkov, B.: Colony development of laser printed eukaryotic (yeast and microalga) microorganisms in co-culture. In: International Journal of Bioprinting 2 (2016), Nr. 2, S. 37-43. DOI:
https://doi.org/10.18063/IJB.2016.02.001
Zusammenfassung: |
Laser Induced Forward Transfer (LIFT) bioprinting is one of a group of techniques that have been largely applied for printing mammalian cells so far. Bioprinting allows precise placement of viable cells in a defined matrix with the aim of directed three-dimensional development of tissues. In this study, laser bioprinting is used to precisely place eukaryotic microorganisms in specific patterns that allow growth and microscopic observation of the organisms' micro-colonies. Saccharomyces cerevisiae var. bayanus and Chlorella vulgaris are used as model organisms for this purpose. Growth and development of the micro-colonies are studied via confocal microscopy and the colonies' growth rates are determined by image analysis. The developed protocols for printing of microorganisms and growth-rate deter-mination of the micro-colonies are very promising for future studies of colony growth and development.
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Lizenzbestimmungen: |
CC BY-NC 4.0 Unported - https://creativecommons.org/licenses/by-nc/4.0/
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Publikationstyp: |
Article |
Publikationsstatus: |
publishedVersion |
Erstveröffentlichung: |
2016 |
Schlagwörter (englisch): |
Bioprinting, Chlorella vulgaris, Growth rates of micro-colonies, Laser-induced forward transfer, Printing of microorganisms, Saccharomyces cerevisiae
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Fachliche Zuordnung (DDC): |
570 | Biowissenschaften, Biologie, 610 | Medizin, Gesundheit
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