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Co-Immobilization of RizA Variants with Acetate Kinase for the Production of Bioactive Arginyl Dipeptides

Bordewick, Sven; Berger, Ralf G.; Ersoy, Franziska (Basel : MDPI, 2022)

The biocatalytic system comprised of RizA and acetate kinase (AckA) combines the specific synthesis of bioactive arginyl dipeptides with efficient ATP regeneration. Immobilization of this coupled enzyme system was performed ...
Mutagenesis of the l-Amino Acid Ligase RizA Increased the Production of Bioactive Dipeptides

Bordewick, Sven; Berger, Ralf G.; Ersoy, Franziska (Basel : MDPI, 2021)

The l-amino acid ligase RizA from B. subtilis selectively synthesizes dipeptides containing an N-terminal arginine. Many arginyl dipeptides have salt-taste enhancing properties while Arg-Phe has been found to have an ...
Side effects of chaperone gene co-expression in recombinant protein production

Martínez-Alonso, Monica; García-Fruitós, Elena; Ferrer-Miralles, Neus; Rinas, Ursula; Villaverde, Antonio (London : BioMed Central Ltd., 2010)

Insufficient availability of molecular chaperones is observed as a major bottleneck for proper protein folding in recombinant protein production. Therefore, co-production of selected sets of cell chaperones along with ...
Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli

Rueda, Fabian; Céspedes, Maria Virtudes; Sánchez-Chardi, Alejandro; Seras-Franzoso, Joaquin; Pesarrodona, Mireia; Ferrer-Miralles, Neus; Vázquez, Esther; Rinas, Ursula; Unzueta, Ugutz; Mamat, Uwe; Mangues, Ramon; García-Fruitós, Elena; Villaverde, Antonio (London : BioMed Central Ltd., 2016)

Background: Production of recombinant drugs in process-friendly endotoxin-free bacterial factories targets to a lessened complexity of the purification process combined with minimized biological hazards during product ...

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Browsing by Subject "protein engineering"

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