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Browsing by Subject "protein binding"

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The Arabidopsis class II sirtuin is a lysine deacetylase and interacts with mitochondrial energy metabolism

König, Ann-Christine; Hartl, Markus; Pham, Phuong Anh; Laxa, Miriam; Boersema, Paul J.; Orwat, Anne; Kalitventseva, Ievgeniia; Plöchinger, Magdalena; Braun, Hans-Peter; Leister, Dario; Mann, Matthias; Wachter, Andreas; Fernie, Alisdair R.; Finkemeier, Iris (Rockville, Md. : American Society of Plant Physiologists, 2014)

The posttranslational regulation of proteins by lysine (Lys) acetylation has recently emerged to occur not only on histones, but also on organellar proteins in plants and animals. In particular, the catalytic activities ...
Comparison of colorimetric methods for the quantification of model proteins in aqueous two-phase systems

Glyk, Anna; Heinisch, Sandra L.; Scheper, Thomas; Beutel, Sascha (Cambridge, MA : Elsevier Academic Press, 2015)

In the current study, the quantification of different model proteins in the presence of typical aqueous two-phase system components was investigated by using the Bradford and bicinchoninic acid (BCA) assays. Each phase-forming ...
Downstream processing of high chain length polysialic acid using membrane adsorbers and clay minerals for application in tissue engineering

Bice, Ismet; Celik, Hilal; Wolff, Christopher; Beutel, Sascha; Zahid, Maria; Hitzmann, Bernd; Rinas, Ursula; Kasper, Cornelia; Gerardy-Schahn, Rita; Scheper, Thomas (Weinheim : Wiley-VCH Verlag, 2013)

Polysialic acid (polySia) is a carbohydrate polymer of varying chain length. It is a promising scaffold material for tissue engineering. In this work, high chain length polySia was produced by an Escherichia coli K1 strain ...
Identification of genes differentially expressed in a resistant reaction to Mycosphaerella pinodes in pea using microarray technology

Fondevilla, Sara; Küster, Helge; Krajinski, Franziska; Cubero, José I.; Rubiales, Diego (London : BioMed Central Ltd., 2011)

Background: Ascochyta blight, caused by Mycosphaerella pinodes is one of the most important pea pathogens. However, little is known about the genes and mechanisms of resistance acting against M. pinodes in pea. Resistance ...
Inositol pyrophosphates promote the interaction of SPX domains with the coiled-coil motif of PHR transcription factors to regulate plant phosphate homeostasis

Ried, Martina K.; Wild, Rebekka; Zhu, Jinsheng; Pipercevic, Joka; Sturm, Kristina; Broger, Larissa; Harmel, Robert K.; Abriata, Luciano A.; Hothorn, Ludwig A.; Fiedler, Dorothea; Hiller, Sebastian; Hothorn, Michael (London : Nature Publishing Group, 2021)

Phosphorus is an essential nutrient taken up by organisms in the form of inorganic phosphate (Pi). Eukaryotes have evolved sophisticated Pi sensing and signaling cascades, enabling them to stably maintain cellular Pi ...
A potential late stage intermediate of twin-arginine dependent protein translocation in Escherichia coli

Geise, Hendrik; Heidrich, Eyleen Sabine; Nikolin, Christoph Stefan; Mehner-Breitfeld, Denise; Brüser, Thomas (Lausanne : Frontiers Media S.A., 2019)

The twin-arginine translocation (Tat) system transports folded proteins across membranes of prokaryotes, plant plastids, and some mitochondria. According to blue-native polyacrylamide gel electrophoresis after solubilization ...
Side effects of chaperone gene co-expression in recombinant protein production

Martínez-Alonso, Monica; García-Fruitós, Elena; Ferrer-Miralles, Neus; Rinas, Ursula; Villaverde, Antonio (London : BioMed Central Ltd., 2010)

Insufficient availability of molecular chaperones is observed as a major bottleneck for proper protein folding in recombinant protein production. Therefore, co-production of selected sets of cell chaperones along with ...
Structural Basis for Regulation of the Opposing (p)ppGpp Synthetase and Hydrolase within the Stringent Response Orchestrator Rel

Pausch, Patrick; Abdelshahid, Maha; Steinchen, Wieland; Schäfer, Heinrich; Gratani, Fabio Lino; Freibert, Sven-Andreas.; Wolz, Christiane; Turgay, Kürşad; Wilson, Daniel N.; Bange, Gert (New York, NY : Elsevier, 2020)

The stringent response enables metabolic adaptation of bacteria under stress conditions and is governed by RelA/SpoT Homolog (RSH)-type enzymes. Long RSH-type enzymes encompass an N-terminal domain (NTD) harboring the ...
A structural model of the cytochrome c reductase/oxidase supercomplex from yeast mitochondria

Heinemeyer, Jesco; Braun, Hans-Peter; Boekema, Egbert J.; Kouřil, Roman (Bethesda, Md. : ASBMB Publications, 2007)

Mitochondrial respiratory chain complexes are arranged in supercomplexes within the inner membrane. Interaction of cytochrome c reductase (complex III) and cytochrome c oxidase (complex IV) was investigated in Saccharomyces ...
SUMO-fusion, purification, and characterization of a (+)-zizaene synthase from Chrysopogon zizanioides

Hartwig, Steffen; Frister, Thore; Alemdar, Semra; Li, Zhaopeng; Scheper, Thomas; Beutel, Sascha (Cambridge, MA : Elsevier Academic Press, 2015)

An uncharacterized plant cDNA coding for a polypeptide presumably having sesquiterpene synthase activity, was expressed in soluble and active form. Two expression strategies were evaluated in Escherichia coli. The enzyme ...

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