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Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: Efficiency and cytotoxicity

Durán, Maria Carolina; Willenbrock, Saskia; Barchanski, Annette; Müller, Jessika-M.V.; Maiolini, Arianna; Soller, Jan T.; Barcikowski, Stephan; Nolte, Ingo; Feige, Karsten; Murua Escobar, Hugo (London : Biomed Central, 2011)

Background: Reproducibly high transfection rates with low methodology-induced cytotoxic side effects are essential to attain the required effect on targeted cells when exogenous DNA is transfected. Different approaches and ...
Gene Electrotransfer into Mammalian Cells Using Commercial Cell Culture Inserts with Porous Substrate

Vindiš, Tina; Blažič, Anja; Khayyat, Diaa; Potočnik, Tjaša; Sachdev, Shaurya; Rems, Lea (Basel : MDPI, 2022)

Gene electrotransfer is one of the main non-viral methods for intracellular delivery of plasmid DNA, wherein pulsed electric fields are used to transiently permeabilize the cell membrane, allowing enhanced transmembrane ...
A potential late stage intermediate of twin-arginine dependent protein translocation in Escherichia coli

Geise, Hendrik; Heidrich, Eyleen Sabine; Nikolin, Christoph Stefan; Mehner-Breitfeld, Denise; Brüser, Thomas (Lausanne : Frontiers Media S.A., 2019)

The twin-arginine translocation (Tat) system transports folded proteins across membranes of prokaryotes, plant plastids, and some mitochondria. According to blue-native polyacrylamide gel electrophoresis after solubilization ...
Recombinant protein production provoked accumulation of ATP, fructose-1,6-bisphosphate and pyruvate in E. coli K12 strain TG1

Weber, Jan; Li, Zhaopeng; Rinas, Ursula (London : Biomed Central, 2021)

Background: Recently it was shown that production of recombinant proteins in E. coli BL21(DE3) using pET based expression vectors leads to metabolic stress comparable to a carbon overfeeding response. Opposite to original ...
Whole-genome sequences of two new caballeronia strains isolated from cryoturbated peat circles of the permafrost-affected eastern european tundra

Hetz, Stefanie A.; Poehlein, Anja; Horn, Marcus A. (Washington, DC : American Society for Microbiology, 2020)

Annotated genomes of Caballeronia strains SBC1 and SBC2 from acidic permafrost suggest a new species with a facultative lifestyle via oxygen and nitrate respiration. Thus, a contribution to nitrogen cycling in cold and ...

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