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3-hydroxyisobutyrate dehydrogenase is involved in both, valine and isoleucine degradation in arabidopsis thaliana

Schertl, Peter; Danne, Lennart; Braun, Hans-Peter (Rockville, Md. : American Society of Plant Physiologists, 2017)

In plants, amino acid catabolism is especially relevant in metabolic stress situations (e.g. limited carbohydrate availability during extended darkness). Under these conditions, amino acids are used as alternative substrates ...
An unusual overrepresentation of genetic factors related to iron homeostasis in the genome of the fluorescent Pseudomonas sp. ABC1

Valenzuela-Heredia, Daniel; Henríquez-Castillo, Carlos; Donoso, Raúl; Lavín, Paris; Ringel, Michael T.; Brüser, Thomas; Campos, José Luis (Oxford : Wiley-Blackwell, 2021)

Members of the genus Pseudomonas inhabit diverse environments, such as soil, water, plants and humans. The variability of habitats is reflected in the diversity of the structure and composition of their genomes. This ...
The Arabidopsis class II sirtuin is a lysine deacetylase and interacts with mitochondrial energy metabolism

König, Ann-Christine; Hartl, Markus; Pham, Phuong Anh; Laxa, Miriam; Boersema, Paul J.; Orwat, Anne; Kalitventseva, Ievgeniia; Plöchinger, Magdalena; Braun, Hans-Peter; Leister, Dario; Mann, Matthias; Wachter, Andreas; Fernie, Alisdair R.; Finkemeier, Iris (Rockville, Md. : American Society of Plant Physiologists, 2014)

The posttranslational regulation of proteins by lysine (Lys) acetylation has recently emerged to occur not only on histones, but also on organellar proteins in plants and animals. In particular, the catalytic activities ...
The carbonic anhydrase domain of plant mitochondrial complex I

Fromm, Steffanie; Senkler, Jennifer; Zabaleta, Eduardo; Peterhänsel, Christoph; Braun, Hans-Peter (Oxford [u.a.] : Wiley-Blackwell, 2016)

The mitochondrial NADH dehydrogenase complex (complex I) consists of several functional domains which independently arose during evolution. In higher plants, it contains an additional domain which includes proteins resembling ...
Cellular plasticity in response to suppression of storage proteins in the Brassica napus embryo

Rolletschek, Hardy; Schwender, Jörg; König, Christina; Chapman, Kent D.; Romsdahl, Trevor; Lorenz, Christin; Braun, Hans-Peter; Denolf, Peter; van Audenhove, Katrien; Munz, Eberhard; Heinzel, Nicolas; Ortleb, Stefan; Rutten, Twan; McCorkle, Sean; Borysyuk, Taras; Guendel, André; Shi, Hai; Auwermeulen, Michiel Vander; Bourot, Stephane; Borisjuk, Ljudmilla (Rockville, Md. : American Society of Plant Physiologists, 2020)

The tradeoff between protein and oil storage in oilseed crops has been tested here in oilseed rape (Brassica napus) by analyzing the effect of suppressing key genes encoding protein storage products (napin and cruciferin). ...
Characterisation of a Recombinant Patchoulol Synthase Variant for Biocatalytic Production of Terpenes

Frister, Thore; Hartwig, Steffen; Alemdar, Semra; Schnatz, Katharina; Thöns, Laura; Scheper, Thomas; Beutel, Sascha (New York City, NY : Springer Humana Press Inc., 2015)

The patchoulol synthase (PTS) is a multi-product sesquiterpene synthases which is the central enzyme for biosynthesis of patchouli essential oil in the patchouli plant. Sesquiterpene synthases catalyse the formation of ...
Characterization of leaf apoplastic peroxidases and metabolites in Vigna unguiculata in response to toxic manganese supply and silicon

Führs, Hendrik; Götze, Stefanie; Specht, André; Erban, Alexander; Gallien, Sébastien; Heintz, Dimitri; Van Dorsselaer, Alain; Kopka, Joachim; Braun, Hans-Peter; Horst, Walter J. (Oxford : Oxford University Press, 2009)

Previous work suggested that the apoplastic phenol composition and its interaction with apoplastic class III peroxidases (PODs) are decisive in the development or avoidance of manganese (Mn) toxicity in cowpea (Vigna ...
Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: Efficiency and cytotoxicity

Durán, Maria Carolina; Willenbrock, Saskia; Barchanski, Annette; Müller, Jessika-M.V.; Maiolini, Arianna; Soller, Jan T.; Barcikowski, Stephan; Nolte, Ingo; Feige, Karsten; Murua Escobar, Hugo (London : Biomed Central, 2011)

Background: Reproducibly high transfection rates with low methodology-induced cytotoxic side effects are essential to attain the required effect on targeted cells when exogenous DNA is transfected. Different approaches and ...
Complete genome sequence and construction of an infectious full-length cDNA clone of a cucumber vein yellowing virus (CVYV) isolate from Portugal

Cordes, K.; Maiss, E.; Winter, S.; Rose, H. (Wien ; New York, NY : Springer, 2021)

Cucumber vein yellowing virus (CVYV) is a member of the genus Ipomovirus in the family Potyviridae. In the National Center for Biotechnology Information (NCBI) database, three complete genome sequences of CVYV isolates ...
Differential proteomics analysis of Frankliniella occidentalis immune response after infection with Tomato spotted wilt virus (Tospovirus)

Ogada, Pamella Akoth; Kiirika, Leonard Muriithi; Lorenz, Christin; Senkler, Jennifer; Braun, Hans-Peter; Poehling, Hans-Michael (Amsterdam [u.a.] : Elsevier Science, 2017)

Tomato spotted wilt virus (TSWV) is mainly vectored by Frankliniella occidentalis Pergande, and it potentially activates the vector's immune response. However, molecular background of the altered immune response is not ...
A draft genome sequence of the rose black spot fungus Diplocarpon rosae reveals a high degree of genome duplication

Neu, Enzo; Featherston, Jonathan; Rees, Jasper; Debener, Thomas (San Francisco, CA : Public Library of Science, 2017)

Background: Black spot is one of the most severe and damaging diseases of garden roses. We present the draft genome sequence of its causative agent Diplocarpon rosae as a working tool to generate molecular markers and to ...
Enhancing transgenic pea (Pisum sativum L.) resistance against fungal diseases through stacking of two antifungal genes (chitinase and glucanase).

Amian, Awah Anna; Papenbrock, Jutta; Jacobsen, Hans-Jörg; Hassan, Fathi (Abingdon : Taylor and Francis Ltd., 2011)

One way of enhancing and broadening resistance of plants to different biotic and abiotic stresses is to combine transgenes expressing several genes into a single line. This can be done using different strategies such as ...
ICTV virus taxonomy profile: Partitiviridae

Vainio, Eeva J.; Chiba, Sotaro; Ghabrial, Said A.; Maiss, Edgar; Roossinck, Marilyn; Sabanadzovic, Sead; Suzuki, Nobuhiro; Xie, Jiatao; Nibert, Max; Lefkowitz, E.J.; Davison, A.J.; Siddell, S.G.; Smith, D.B.; Orton, R.J.; Simmonds, P.; ICTV Report Consortium (London : Microbiology Society, 2018)

The Partitiviridae is a family of small, isometric, non-enveloped viruses with bisegmented double-stranded (ds) RNA genomes of 3-4.8 kbp. The two genome segments are individually encapsidated. The family has five genera, ...
Identification of genes differentially expressed in a resistant reaction to Mycosphaerella pinodes in pea using microarray technology

Fondevilla, Sara; Küster, Helge; Krajinski, Franziska; Cubero, José I.; Rubiales, Diego (London : BioMed Central Ltd., 2011)

Background: Ascochyta blight, caused by Mycosphaerella pinodes is one of the most important pea pathogens. However, little is known about the genes and mechanisms of resistance acting against M. pinodes in pea. Resistance ...
Inner tegument proteins of Herpes Simplex Virus are sufficient for intracellular capsid motility in neurons but not for axonal targeting

Buch, Anna; Müller, Oliver; Ivanova, Lyudmila; Döhner, Katinka; Bialy, Dagmara; Bosse, Jens B.; Pohlmann, Anja; Binz, Anne; Hegemann, Maike; Nagel, Claus-Henning; Koltzenburg, Martin; Viejo-Borbolla, Abel; Rosenhahn, Bodo; Bauerfeind, Rudolf; Sodeik, Beate (San Francisco, CA : Public Library of Science (PLoS), 2017)

Upon reactivation from latency and during lytic infections in neurons, alphaherpesviruses assemble cytosolic capsids, capsids associated with enveloping membranes, and transport vesicles harboring fully enveloped capsids. ...
Inositol pyrophosphates promote the interaction of SPX domains with the coiled-coil motif of PHR transcription factors to regulate plant phosphate homeostasis

Ried, Martina K.; Wild, Rebekka; Zhu, Jinsheng; Pipercevic, Joka; Sturm, Kristina; Broger, Larissa; Harmel, Robert K.; Abriata, Luciano A.; Hothorn, Ludwig A.; Fiedler, Dorothea; Hiller, Sebastian; Hothorn, Michael (London : Nature Publishing Group, 2021)

Phosphorus is an essential nutrient taken up by organisms in the form of inorganic phosphate (Pi). Eukaryotes have evolved sophisticated Pi sensing and signaling cascades, enabling them to stably maintain cellular Pi ...
Interaction of roses with a biotrophic and a hemibiotrophic leaf pathogen leads to differences in defense transcriptome activation

Neu, Enzo; Domes, Helena Sophie; Menz, Ina; Kaufmann, Helgard; Linde, Marcus; Debener, Thomas (Dordrecht : Springer Netherlands, 2019)

Key message: Transcriptomic analysis resulted in the upregulation of the genes related to common defense mechanisms for black spot and the downregulation of the genes related to photosynthesis and cell wall modification ...
L-amino acid oxidases with specificity for basic L-amino acids in cyanobacteria

Gau, Achim E.; Heindl, Achim; Nodop, Anke; Kahmann, Uwe; Pistorius, Elfriede K. (Berlin : De Gruyter, 2007)

The two closely related fresh water cyanobacteria Synechococcus elongatus PCC 6301 and Synechococcus elongatus PCC 7942 have previously been shown to constitutively express a FAD-containing L-amino acid oxidase with high ...
The life of plant mitochondrial complex I

Braun, Hans-Peter; Binder, Stefan; Brennicke, Axel; Eubel, Holger; Fernie, Alisdair R.; Finkemeier, Iris; Klodmann, Jennifer; König, Ann-Christin; Kühn, Kristina; Meyer, Etienne; Obata, Toshihiro; Schwarzländer, Markus; Takenaka, Mizuka; Zehrmann, Anja (Amsterdam [u.a.] : Elsevier Science, 2014)

The mitochondrial NADH dehydrogenase complex (complex I) of the respiratory chain has several remarkable features in plants: (i) particularly many of its subunits are encoded by the mitochondrial genome, (ii) its mitochondrial ...
Localisation of abundant and organ-specific genes expressed in Rosa hybrida leaves and flower buds by direct in situ RT-PCR

Jedrzejuk, Agata; Mibus, Heiko; Serek, Margrethe (New York, NY : Hindawi Publishing Corporation, 2012)

In situ PCR is a technique that allows specific nucleic acid sequences to be detected in individual cells and tissues. In situ PCR and IS-RT-PCR are elegant techniques that can increase both sensitivity and throughput, but ...

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