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Browsing by Subject "cell viability"

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Adhesion, Vitality and Osteogenic Differentiation Capacity of Adipose Derived Stem Cells Seeded on Nitinol Nanoparticle Coatings

Strauß, Sarah; Neumeister, Anne; Barcikowski, Stephan; Kracht, Dietmar; Kuhbier, Jörn W.; Radtke, Christine; Reimers, Kerstin; Vogt, Peter M. (San Francisco, California, US : PLOS, 2013)

Autologous cells can be used for a bioactivation of osteoimplants to enhance osseointegration. In this regard, adipose derived stem cells (ASCs) offer interesting perspectives in implantology because they are fast and easy ...
EBIO Does Not Induce Cardiomyogenesis in Human Pluripotent Stem Cells but Modulates Cardiac Subtype Enrichment by Lineage-Selective Survival

Jara-Avaca, Monica; Kempf, Henning; Rückert, Michael; Robles-Diaz, Diana; Franke, Annika; de la Roche, Jeanne; Fischer, Martin; Malan, Daniela; Sasse, Philipp; Solodenko, Wladimir; Dräger, Gerald; Kirschning, Andreas; Martin, Ulrich; Zweigerdt, Robert (New York, NY : Springer, 2017)

Subtype-specific human cardiomyocytes (CMs) are valuable for basic and applied research. Induction of cardiomyogenesis and enrichment of nodal-like CMs was described for mouse pluripotent stem cells (mPSCs) in response to ...
Evaluation of the biological tolerability of the starch-based medical device 4DryField® PH in vitro and in vivo a rat model

Poehnert, Daniel; Abbas, Mahmoud; Maegel, Lavinia; Sambale, Franziska; Lavrentieva, Antonina; Kreipe, Hans-Heinrich; Klempnauer, Jürgen; Winny, Markus (London : SAGE Publications Ltd., 2015)

Purpose To evaluate in vitro cytotoxicity/biocompatibility as well as in vivo tolerability of the novel polysaccharide 4DryField® PH, certified for haemostasis and adhesion prevention. Methods In vitro cytotoxicity/viability ...
Gold Nanoparticle Mediated Laser Transfection for Efficient siRNA Mediated Gene Knock Down

Heinemann, Dag; Schomaker, Markus; Kalies, Stefan; Schieck, Maximilian; Carlson, Regina; Murua Escobar, Hugo; Ripken, Tammo; Meyer, Heiko; Heisterkamp, Alexander (San Francisco, California, US : PLOS, 2013)

Laser based transfection methods have proven to be an efficient and gentle alternative to established molecule delivery methods like lipofection or electroporation. Among the laser based methods, gold nanoparticle mediated ...
Investigation of biophysical mechanisms in gold nanoparticle mediated laser manipulation of cells using a multimodal holographic and fluorescence imaging setup

Kalies, Stefan; Antonopoulos, Georgios C.; Rakoski, Mirko S.; Heinemann, Dag; Schomaker, Markus; Ripken, Tammo; Meyer, Heiko (San Francisco, CA : Public Library of Science (PLoS), 2015)

Laser based cell manipulation has proven to be a versatile tool in biomedical applications. In this context, combining weakly focused laser pulses and nanostructures, e.g. gold nanoparticles, promises to be useful for high ...
Magnetic beads enhance adhesion of NIH 3T3 fibroblasts: A proof-of-principle in vitro study for implant-mediated long-term drug delivery to the inner ear

Aliuos, Pooyan; Schulze, Jennifer; Schomaker, Markus; Reuter, Günter; Stolle, Stefan R.O.; Werner, Darja; Ripken, Tammo; Lenarz, Thomas; Warnecke, Athanasia (San Francisco, CA : Public Library of Science (PLoS), 2016)

Introduction Long-term drug delivery to the inner ear may be achieved by functionalizing cochlear implant (CI) electrodes with cells providing neuroprotective factors. However, effective strategies in order to coat implant ...
Murine and human pluripotent stem cell-derived cardiac bodies form contractile myocardial tissue in vitro

Kensah, George; Lara, Angelica Roa; Dahlmann, Julia; Zweigerdt, Robert; Schwanke, Kristin; Hegermann, Jan; Skvorc, David; Gawol, Anke; Azizian, Azadeh; Wagner, Stefan; Maier, Lars S.; Krause, Andreas; Dräger, Gerald; Ochs, Matthias; Haverich, Axel; Gruh, Ina; Martin, Ulrich (Oxford : Oxford University Press, 2013)

AimsWe explored the use of highly purified murine and human pluripotent stem cell (PSC)-derived cardiomyocytes (CMs) to generate functional bioartificial cardiac tissue (BCT) and investigated the role of fibroblasts, ...
Optical and electron microscopy study of laser-based intracellular molecule delivery using peptide-conjugated photodispersible gold nanoparticle agglomerates

Krawinkel, Judith; Richter, Undine; Torres-Mapa, Maria Leilani; Westermann, Martin; Gamrad, Lisa; Rehbock, Christoph; Barcikowski, Stephan; Heisterkamp, Alexander (London : BioMed Central Ltd., 2016)

Background: Cell-penetrating peptides (CPPs) can act as carriers for therapeutic molecules such as drugs and genetic constructs for medical applications. The triggered release of the molecule into the cytoplasm can be ...
Regulatory coiled-coil domains promote head-to-head assemblies of AAA+ chaperones essential for tunable activity control

Carroni, Marta; Franke, Kamila B.; Maurer, Michael; Jäger, Jasmin; Hantke, Ingo; Gloge, Felix; Linder, Daniela; Gremer, Sebastian; Turgay, Kürsad; Bukau, Bernd; Mogk, Axel (Cambridge : eLife Sciences Publications, 2017)

Ring-forming AAA+ chaperones exert ATP-fueled substrate unfolding by threading through a central pore. This activity is potentially harmful requiring mechanisms for tight repression and substrate-specific activation. The ...

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