Auflistung nach Schlagwort "Proteins"

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  • Blue native DIGE as a tool for comparative analyses of protein complexes 
    Heinemeyer, Jesco; Scheibe, Burghardt; Schmitz, Udo K.; Braun, Hans-Peter (New York, NY [u.a.] : Elsevier, 2009)
    Differential gel electrophoresis (DIGE) is based on pre-labeling of different protein fractions and their subsequent co-electrophoresis in a single gel. Cyanine based "CyDye DIGE Fluor minimal dyes" are used for the labeling ...
  • Capturing protein domain structure and function using self-supervision on domain architectures 
    Melidis, Damianos P.; Nejdl, Wolfgang (Basel : MDPI AG, 2021)
    Predicting biological properties of unseen proteins is shown to be improved by the use of protein sequence embeddings. However, these sequence embeddings have the caveat that biological metadata do not exist for each amino ...
  • Carbonic anhydrase subunits form a matrix-exposed domain attached to the membrane arm of mitochondrial complex I in plants 
    Sunderhaus, Stephanie; Dudkina, Natalya V.; Jänsch, Lothar; Klodmann, Jennifer; Heinemeyer, Jesco; Perales, Mariano; Zabaleta, Eduardo; Boekema, Egbert J.; Braun, Hans-Peter (Bethesda, Md. : ASBMB Publications, 2006)
    Complex I of Arabidopsis includes five structurally related subunits representing γ-type carbonic anhydrases termed CA1, CA2, CA3, CAL1, and CAL2. The position of these subunits within complex I was investigated. Direct ...
  • CEDAR, an online resource for the reporting and exploration of complexome profiling data 
    van Strien, Joeri; Haupt, Alexander S.; Schulte, Uwe; Braun, Hans-Peter; Cabrera-Orefice, Alfredo; Choudhary, Jyoti S.; Evers, Felix; Fernandez-Vizarra, Erika; Guerrero-Castillo, Sergio; Kooij, Taco W.A.; Páleníková, Petra; Pardo, Mercedes; Ugalde, Cristina; Wittig, Ilka; Wöhlbrand, Lars; Brandt, Ulrich; Arnold, Susanne; Huynen, Martijn A. (Amsterdam : Elsevier, 2021)
    Complexome profiling is an emerging ‘omics’ approach that systematically interrogates the composition of protein complexes (the complexome) of a sample, by combining biochemical separation of native protein complexes with ...
  • The coenzyme/protein pair and the molecular evolution of life 
    Kirschning, Andreas (London : Soc., 2021)
    Covering: up to 2020 What was first? Coenzymes or proteins? These questions are archetypal examples of causal circularity in living systems. Classically, this "chicken-and-egg"problem was discussed for the macromolecules ...
  • Comparison of colorimetric methods for the quantification of model proteins in aqueous two-phase systems 
    Glyk, Anna; Heinisch, Sandra L.; Scheper, Thomas; Beutel, Sascha (Cambridge, MA : Elsevier Academic Press, 2015)
    In the current study, the quantification of different model proteins in the presence of typical aqueous two-phase system components was investigated by using the Bradford and bicinchoninic acid (BCA) assays. Each phase-forming ...
  • Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: Efficiency and cytotoxicity 
    Durán, Maria Carolina; Willenbrock, Saskia; Barchanski, Annette; Müller, Jessika-M.V.; Maiolini, Arianna; Soller, Jan T.; Barcikowski, Stephan; Nolte, Ingo; Feige, Karsten; Murua Escobar, Hugo (London : Biomed Central, 2011)
    Background: Reproducibly high transfection rates with low methodology-induced cytotoxic side effects are essential to attain the required effect on targeted cells when exogenous DNA is transfected. Different approaches and ...
  • Computer-Aided Design and Synthesis of a New Class of PEX14 Inhibitors: Substituted 2,3,4,5-Tetrahydrobenzo[F][1,4]oxazepines as Potential New Trypanocidal Agents 
    Fino, Roberto; Lenhart, Dominik; Kalel, Vishal C.; Softley, Charlotte A.; Napolitano, Valeria; Byrne, Ryan; Schliebs, Wolfgang; Dawidowski, Maciej; Erdmann, Ralf; Sattler, Michael; Schneider, Gisbert; Plettenburg, Oliver; Popowicz, Grzegorz M. (Washington, DC : American Chemical Society, 2021)
    African and American trypanosomiases are estimated to affect several million people across the world, with effective treatments distinctly lacking. New, ideally oral, treatments with higher efficacy against these diseases ...
  • Electrospray sample injection for single-particle imaging with x-ray lasers 
    Bielecki, Johan; Hantke, Max F.; Daurer, Benedikt J.; Reddy, Hemanth K.N.; Hasse, Dirk; Larsson, Daniel S.D.; Gunn, Laura H.; Svenda, Martin; Iwan, Bianca (Washington : American Association for the Advancement of Science (A A A S), 2019)
    The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite ...
  • GelMap - A novel software tool for building and presenting proteome reference maps 
    Rode, Christina; Senkler, Michael; Klodmann, Jennifer; Winkelmann, Traud; Braun, Hans-Peter (New York, NY [u.a.] : Elsevier, 2011)
    Protein separation by two-dimensional gel electrophoresis is of central importance for proteomics. Upon combination with systematic protein identifications by mass spectrometry, large data sets are routinely generated in ...
  • The gene space of European mistletoe (Viscum album) 
    Schröder, Lucie; Hohnjec, Natalija; Senkler, Michael; Senkler, Jennifer; Küster, Helge; Braun, Hans-Peter (Oxford [u.a.] : Wiley-Blackwell, 2022)
    European mistletoe (Viscum album) is a hemiparasitic flowering plant that is known for its very special life cycle and extraordinary biochemical properties. Particularly, V. album has an unusual mode of cellular respiration ...
  • Heterologous Production of Fungal Maleidrides Reveals the Cryptic Cyclization Involved in their Biosynthesis 
    Williams, Katherine; Szwalbe, Agnieszka J.; Mulholland, Nicholas P.; Vincent, Jason L.; Bailey, Andrew M.; Willis, Christine L.; Simpson, Thomas J.; Cox, Russell J. (Weinheim : Wiley-VCH Verlag, 2016)
    Fungal maleidrides are an important family of bioactive secondary metabolites that consist of 7, 8, or 9-membered carbocycles with one or two fused maleic anhydride moieties. The biosynthesis of byssochlamic acid (a ...
  • Identification of RNA Base Pairs and Complete Assignment of Nucleobase Resonances by Proton-Detected Solid-State NMR Spectroscopy at 100 kHz MAS 
    Aguion, Philipp Innig; Kirkpatrick, John; Carlomagno, Teresa; Marchanka, Alexander (Weinheim : Wiley-VCH, 2021)
    Knowledge of RNA structure, either in isolation or in complex, is fundamental to understand the mechanism of cellular processes. Solid-state NMR (ssNMR) is applicable to high molecular-weight complexes and does not require ...
  • The influence of structural gradients in large pore organosilica materials on the capabilities for hosting cellular communities 
    Bronner, Hannah; Holzer, Anna-Katharina; Finke, Alexander; Kunkel, Marius; Marx, Andreas; Leist, Marcel; Polarz, Sebastian (London : RSC Publishing, 2020)
    Cells exist in the so-called extracellular matrix (ECM) in their native state, and numerous future applications require reliable and potent ECM-mimics. A perspective, which goes beyond ECM emulation, is the design of a ...
  • Input of easily available organic C and N stimulates microbial decomposition of soil organic matter in arctic permafrost soil 
    Wild, Birgit; Schnecker, Jörg; Alves, Ricardo J. Eloy; Barsukov, Pavel; Barta, Jiri; Čapek, Petr; Gentsch, Norman; Gittel, Antje; Guggenberger, Georg; Lashchinskiy, Nikolay; Mikutta, Robert; Rusalimova, Olga; Šantrůčková, Hana; Shibistova, Olga; Urich, Tim; Watzka, Margarete; Zrazhevskaya, Galina; Richter, Andreas (London : Elsevier Ltd., 2014)
    Rising temperatures in the Arctic can affect soil organic matter (SOM) decomposition directly and indirectly, by increasing plant primary production and thus the allocation of plant-derived organic compounds into the soil. ...
  • The native structure and composition of the cruciferin complex in brassica napus 
    Nietzel, Thomas; Dudkina, Natalya V.; Haase, Christin; Denolf, Peter; Semchonok, Dmitry A.; Boekema, Egbert J.; Braun, Hans-Peter; Sunderhaus, Stephanie (Bethesda, Md. : ASBMB Publications, 2013)
    Globulins are an important group of seed storage proteins in dicotyledonous plants. They are synthesized during seed development, assembled into very compact protein complexes, and finally stored in protein storage vacuoles ...
  • Nitrogen dynamics in Turbic Cryosols from Siberia and Greenland 
    Wild, Birgit; Schnecker, Jörg; Bárta, Jiri; Čapek, Petr; Guggenberger, Georg; Hofhansl, Florian; Kaiser, Christina; Lashchinskiy, Nikolay; Mikutta, Robert; Mooshammer, Maria; Šantrůčková, Hana; Shibistova, Olga; Urich, Tim; Zimov, Sergey A.; Richter, Andreas (Amsterdam : Elsevier, 2013)
    Turbic Cryosols (permafrost soils characterized by cryoturbation, i.e., by mixing of soil layers due to freezing and thawing) are widespread across the Arctic, and contain large amounts of poorly decomposed organic material ...
  • Packaging protein drugs as bacterial inclusion bodies for therapeutic applications 
    Villaverde, Antonio; García-Fruitós, Elena; Rinas, Ursula; Seras-Franzoso, Joaquin; Kosoy, Ana; Corchero, Josè Luis; Vázquez, Esther (London : BioMed Central Ltd., 2012)
    A growing number of insights on the biology of bacterial inclusion bodies (IBs) have revealed intriguing utilities of these protein particles. Since they combine mechanical stability and protein functionality, IBs have ...
  • Polysialic acid production using Escherichia coli K1 in a disposable bag reactor 
    de Vries, Ingo; Busse, Christoph; Kopatz, Jens; Neumann, Harald; Beutel, Sascha; Scheper, Thomas (Weinheim : Wiley-VCH Verlag, 2017)
    Polysialic acid (polySia), consisting of α-(2,8)-linked N-acetylneuraminic acid monomers plays a crucial role in many biological processes. This study presents a novel process for the production of endogenous polySia using ...
  • Purification and partial characterization of an extracellular melanoprotein from the fungus Venturia inaequalis 
    Singh, P.; Piotrowski, M.; Gau, A.E. (Berlin : De Gruyter, 2005)
    The fungus Venturia inaequalis clone No. 36 isolated from Malus domestica cv. Gloster excretes a melanoprotein of 36 kDa in relatively high amounts during growth in liquid culture. The protein was isolated from the culture ...