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Characterization of the bifunctional cytochrome c reductase-processing peptidase complex from potato mitochondria

Emmermann, M.; Braun, Hans-Peter; Arretz, M.; Schmitz, U.K. (Bethesda, Md. : ASBMB Publications, 1993)

In potato, cytochrome c reductase, a protein complex of the respiratory chain, exhibits processing activity toward mitochondrial precursor proteins. One of the two cooperating components of the processing peptidase was ...
Co-transfection of plasmid DNA and laser-generated gold nanoparticles does not disturb the bioactivity of GFP-HMGB1 fusion protein

Petersen, Svea; Soller, Jan T.; Wagner, Siegfried; Richter, Andreas; Bullerdiek, Jörn; Nolte, Ingo; Barcikowski, Stephan; Murua Escobar, Hugo (London : Biomed Central, 2009)

Ultrashort pulsed laser ablation in liquids represents a powerful tool for the generation of pure gold nanoparticles (AuNPs) avoiding chemical precursors and thereby making them especially interesting for biomedical ...
Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: Efficiency and cytotoxicity

Durán, Maria Carolina; Willenbrock, Saskia; Barchanski, Annette; Müller, Jessika-M.V.; Maiolini, Arianna; Soller, Jan T.; Barcikowski, Stephan; Nolte, Ingo; Feige, Karsten; Murua Escobar, Hugo (London : Biomed Central, 2011)

Background: Reproducibly high transfection rates with low methodology-induced cytotoxic side effects are essential to attain the required effect on targeted cells when exogenous DNA is transfected. Different approaches and ...
Freeze-drying of mammalian cells using trehalose: Preservation of DNA integrity

Zhang, Miao; Oldenhof, Harriette; Sydykov, Bulat; Bigalk, Judith; Sieme, Harald; Wolkers, Willem F. (London : Nature Publishing Group, 2017)

The aim of this study was to investigate preservation of biomolecular structures, particularly DNA, in freeze-dried fibroblasts, after loading with trehalose via freezing-induced uptake. Cells were freeze-dried with trehalose ...
Immunogenicity of intensively decellularized equine carotid arteries is conferred by the extracellular matrix protein collagen type VI

Boeer, Ulrike; Buettner, Falk F.R.; Klingenberg, Melanie; Antonopoulos, Georgios C.; Meyer, Heiko; Haverich, Axel; Wilhelmi, Mathias (San Francisco, CA : Public Library of Science (PLoS), 2014)

The limited biocompatibility of decellularized scaffolds is an ongoing challenge in tissue engineering. Here, we demonstrate the residual immunogenicity of an extensively decellularized equine carotid artery (dEAC ...
The Impact of Photobleaching on Microarray Analysis

Haar, Marcel von der; Preuss, John-Alexander; Haar, Kathrin von der; Lindner, Patrick; Scheper, Thomas; Stahl, Frank (Basel : MDPI AG, 2015)

DNA-Microarrays have become a potent technology for high-throughput analysis of genetic regulation. However, the wide dynamic range of signal intensities of fluorophore-based microarrays exceeds the dynamic range of a ...
Inositol pyrophosphates promote the interaction of SPX domains with the coiled-coil motif of PHR transcription factors to regulate plant phosphate homeostasis

Ried, Martina K.; Wild, Rebekka; Zhu, Jinsheng; Pipercevic, Joka; Sturm, Kristina; Broger, Larissa; Harmel, Robert K.; Abriata, Luciano A.; Hothorn, Ludwig A.; Fiedler, Dorothea; Hiller, Sebastian; Hothorn, Michael (London : Nature Publishing Group, 2021)

Phosphorus is an essential nutrient taken up by organisms in the form of inorganic phosphate (Pi). Eukaryotes have evolved sophisticated Pi sensing and signaling cascades, enabling them to stably maintain cellular Pi ...
An Introduction to MPEG-G: The First Open ISO/IEC Standard for the Compression and Exchange of Genomic Sequencing Data

Voges, Jan; Hernaez, Mikel; Mattavelli, Marco; Ostermann, Jorn (New York, NY [u.a.] : Inst., 2021)

The development and progress of high-throughput sequencing technologies have transformed the sequencing of DNA from a scientific research challenge to practice. With the release of the latest generation of sequencing ...
Laser ablation-based one-step generation and bio-functionalization of gold nanoparticles conjugated with aptamers

Walter, Johanna-Gabriela; Petersen, Svea; Stahl, Frank; Scheper, Thomas; Barcikowski, Stephan (London : Biomed Central Ltd., 2010)

Background: Bio-conjugated nanoparticles are important analytical tools with emerging biological and medical applications. In this context, in situ conjugation of nanoparticles with biomolecules via laser ablation in an ...
Optimization of cyanine dye stability and analysis of FRET interaction on DNA microarrays

Haar, Marcel von der; Heuer, Christopher; Pähler, Martin; Haar, Kathrin von der; Lindner, Patrick; Scheper, Thomas; Stahl, Frank (Basel : MDPI AG, 2016)

The application of DNA microarrays for high throughput analysis of genetic regulation is often limited by the fluorophores used as markers. The implementation of multi-scan techniques is limited by the fluorophores’ ...
Polyethylenimine-mediated gene delivery into human bone marrow mesenchymal stem cells from patients

Wang, Weiwei; Li, Wenzhong; Ou, Lailiang; Flick, Eva; Mark, Peter; Nesselmann, Catharina; Lux, Cornelia A.; Gatzen, Hanz-Heinrich; Kaminski, Alexander; Liebold, Andreas; Lützow, Karola; Lendlein, Andreas; Li, Ren-Ke; Steinhoff, Gustav; Ma, Nan (Chichester : Wiley-Blackwell Publishing Ltd., 2011)

Transplantation of mesenchymal stem cells (MSCs) derived from adult bone marrow has been proposed as a potential therapeutic approach for post-infarction left ventricular (LV) dysfunction. However, age-related functional ...
Propidium monoazide pretreatment on a 3D-printed microfluidic device for efficient PCR determination of 'live: Versus dead' microbial cells

Zhu, Yanzhe; Huang, Xiao; Xie, Xing; Bahnemann, Janina; Lin, Xingyu; Wu, Xunyi; Wang, Siwen; Hoffmann, Michael R. (Cambridge : Royal Society of Chemistry, 2018)

Waterborne microbial pathogen detection via nucleic acid analysis on portable microfluidic devices is a growing area of research, development, and application. Traditional polymerase chain reaction (PCR)-based nucleic acid ...
Pyrimidine catabolism is required to prevent the accumulation of 5-methyluridine in RNA

Gao, Shangyu; Sun, Yu; Chen, Xiaoguang; Zhu, Changhua; Liu, Xiaoye; Wang, Wenlei; Gan, Lijun; Lu, Yanwu; Schaarschmidt, Frank; Herde, Marco; Witte, Claus-Peter; Chen, Mingjia (Oxford : Oxford Univ. Press, 2023)

5-Methylated cytosine is a frequent modification in eukaryotic RNA and DNA influencing mRNA stability and gene expression. Here we show that free 5-methylcytidine (5mC) and 5-methyl-2′-deoxycytidine are generated from ...
Restriction fragment length polymorphisms of different DNA regions as genetic markers in the hoverfly Episyrphus balteatus (Diptera: Syrphidae)

Hondelmann, P.; Borgemeister, C.; Poehling, Hans-Michael (Cambridge : Cambridge University Press, 2005)

A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis using mitochondrial (A + T-rich region; mtDNA) and genomic (zen-region; nDNA) DNA was performed on 182 female individuals of Episyrphus ...
A study of gene expression of Saccharomyces cerevisiae in oscillating continous cultures using DNA microarray technology

Khalil Ahmed, Ahmed Abd Allah (Hannover : Gottfried Wilhelm Leibniz Universität Hannover, 2008)

[no abstract]
Three-dimensional cell growth on structures fabricated from ORMOCER® by two-photon polymerization technique

Schlie, Sabrina; Ngezahayo, Anaclet; Ovsianikov, Aleksandr; Fabian, Tilman; Kolb, Hans-Albert; Haferkamp, Heinz; Chichkov, Boris (London : SAGE Publications Ltd., 2007)

Two-photon polymerization technique was applied to generate three-dimensional (3D) scaffold-like structures using the photosensitive organic-inorganic hybrid polymer ORMOCER®. The structures were studied with respect to ...
Transcriptional dynamics of the developing sweet cherry (Prunus avium L.) fruit: sequencing, annotation and expression profiling of exocarp-associated genes

Alkio, Merianne; Jonas, Uwe; Declercq, Myriam; van Nocker, Steven; Knoche, Moritz (London : Macmillan Publishers Limited, 2014)

The exocarp, or skin, of fleshy fruit is a specialized tissue that protects the fruit, attracts seed dispersing fruit eaters, and has large economical relevance for fruit quality. Development of the exocarp involves regulated ...
Whole-genome sequences of two new caballeronia strains isolated from cryoturbated peat circles of the permafrost-affected eastern european tundra

Hetz, Stefanie A.; Poehlein, Anja; Horn, Marcus A. (Washington, DC : American Society for Microbiology, 2020)

Annotated genomes of Caballeronia strains SBC1 and SBC2 from acidic permafrost suggest a new species with a facultative lifestyle via oxygen and nitrate respiration. Thus, a contribution to nitrogen cycling in cold and ...

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