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Browsing by Subject "3D cell culture"

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3D-Printed Microfluidic Perfusion System for Parallel Monitoring of Hydrogel-Embedded Cell Cultures

Meyer, Katharina V.; Winkler, Steffen; Lienig, Pascal; Dräger, Gerald; Bahnemann, Janina (Basel : MDPI, 2023)

The use of three-dimensional (3D) cell cultures has become increasingly popular in the contexts of drug discovery, disease modelling, and tissue engineering, as they aim to replicate in vivo-like conditions. To achieve ...
Comparative analysis of hypoxic response of human microvascular and umbilical vein endothelial cells in 2D and 3D cell culture systems

Dienemann, Sandra; Schmidt, Vanessa; Fleischhammer, Tabea; Mueller, Julia H.; Lavrentieva, Antonina (New York, NY [u.a.] : Wiley-Liss, 2023)

In vitro cultivation conditions play a crucial role in cell physiology and the cellular response to external stimuli. Oxygen concentrations represent an essential microenvironmental factor influencing cell physiology and ...
Dynamic cultivation of human mesenchymal stem/stromal cells for the production of extracellular vesicles in a 3D bioreactor system

Almeria, Ciarra; Weiss, René; Keck, Maike; Weber, Viktoria; Kasper, Cornelia; Egger, Dominik (Dordrecht [u.a.] : Springer Science + Business Media B.V, 2024)

Purpose: 3D cell culture and hypoxia have been demonstrated to increase the therapeutic effects of mesenchymal stem/stromal cells (MSCs)-derived extracellular vesicles (EVs). In this study, a process for the production of ...
Gelatin-methacryloyl (GelMA) formulated with human platelet lysate supports mesenchymal stem cell proliferation and differentiation and enhances the hydrogel’s mechanical properties

Lavrentieva, Antonina; Kirsch, Marline; Birnstein, Luise; Pepelanova, Iliyana; Handke, Wiebke; Rach, Jessica; Seltsam, Axel; Scheper, Thomas (Basel : MDPI AG, 2019)

Three-dimensional (3D) cell culture is a major focus of current research, since cultivation under physiological conditions provides more reliable information about in vivo cell behavior. 3D cell cultures are used in basic ...
Gelatin-methacryloyl (GelMA) hydrogels with defined degree of functionalization as a versatile toolkit for 3D cell culture and extrusion bioprinting

Pepelanova, Iliyana; Kruppa, Katharina; Scheper, Thomas; Lavrentieva, Antonina (Basel : MDPI AG, 2018)

Gelatin-methacryloyl (GelMA) is a semi-synthetic hydrogel which consists of gelatin derivatized with methacrylamide and methacrylate groups. These hydrogels provide cells with an optimal biological environment (e.g., RGD ...
Impact of source and manufacturing of collagen matrices on fibroblast cell growth and platelet aggregation

Böhm, Stefanie; Strauß, Christine; Stoiber, Stefan; Kasper, Cornelia; Charwat, Verena (Basel : MDPI AG, 2017)

Collagen is a main component of the extracellular matrix. It is often used in medical applications to support tissue regeneration, hemostasis, or wound healing. Due to different sources of collagen, the properties and ...
Live reporting for hypoxia : Hypoxia sensor–modified mesenchymal stem cells as in vitro reporters

Schmitz, Carola; Pepelanova, Iliyana; Seliktar, Dror; Potekhina, Ekaterina; Belousov, Vsevolod V.; Scheper, Thomas; Lavrentieva, Antonina (New York, NY [u.a.] : Wiley, 2020)

Natural oxygen gradients occur in tissues of biological organisms and also in the context of three-dimensional (3D) in vitro cultivation. Oxygen diffusion limitation and metabolic oxygen consumption by embedded cells produce ...
Nachhaltige in vitro Kultivierungsansätze in der Zellkulturtechnik: Xenofreie Kultivierungsoptimierung mit Thrombozytenlysat

Kirsch, Hanna Marline (Hannover : Institutionelles Repositorium der Leibniz Universität Hannover, 2021)

Zweidimensionale (2D) oder dreidimensionale (3D) in vitro Kultivierungen vieler Säugetierzellen erfordern eine Supplementierung mit Serum. In klinischen Studien für die regenerative Medizin werden vermehrt mesenchymale ...
Optimization of the cultivation conditions of mesenchymal stem cells with regard to media supplementation, the complexity of the cell construct, and the influence of oxygen

Lavrentieva, Antonina (Hannover : Institutionelles Repositorium der Leibniz Universität Hannover, 2022)

Regenerative medicine aims to not only treat, but also cure diseases and pathological conditions. The utilization of human cells in cell-based therapies raises new hopes for achieving this, while at the same time bringing ...
Physiologic isolation and expansion of human mesenchymal stem/stromal cells for manufacturing of cell-based therapy products

Egger, Dominik; Lavrentieva, Antonina; Kugelmeier, Patrick; Kasper, Cornelia (Weinheim : Wiley-VCH, 2021)

The utilization of mesenchymal stem/stromal cells raises new hopes in treatment of diseases and pathological conditions, while at the same time bringing immense challenges for researchers, manufacturers and physicians. It ...
Solide 3D Hydrogelsysteme zur Kultivierung tierischer Zellen

Rüdinger, Ferdinand Martin (Hannover : Gottfried Wilhelm Leibniz Universität Hannover, 2015)

[no abstract]
Studies on oxygen availability and the creation of natural and artificial oxygen gradients in gelatin-methacryloyl hydrogel 3D cell culture

Schmitz, Carola; Pepelanova, Iliyana; Ude, Christian; Lavrentieva, Antonina (Hoboken, NJ [u.a.] : Wiley, 2022)

Three-dimensional (3D) cultivation platforms allow the creation of cell models, which more closely resemble in vivo-like cell behavior. Therefore, 3D cell culture platforms have started to replace conventional two-dimensional ...

Now showing items 1-12 of 12

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