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Browsing by Subject "genetic transfection"

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Co-transfection of plasmid DNA and laser-generated gold nanoparticles does not disturb the bioactivity of GFP-HMGB1 fusion protein

Petersen, Svea; Soller, Jan T.; Wagner, Siegfried; Richter, Andreas; Bullerdiek, Jörn; Nolte, Ingo; Barcikowski, Stephan; Murua Escobar, Hugo (London : Biomed Central, 2009)

Ultrashort pulsed laser ablation in liquids represents a powerful tool for the generation of pure gold nanoparticles (AuNPs) avoiding chemical precursors and thereby making them especially interesting for biomedical ...
Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: Efficiency and cytotoxicity

Durán, Maria Carolina; Willenbrock, Saskia; Barchanski, Annette; Müller, Jessika-M.V.; Maiolini, Arianna; Soller, Jan T.; Barcikowski, Stephan; Nolte, Ingo; Feige, Karsten; Murua Escobar, Hugo (London : Biomed Central, 2011)

Background: Reproducibly high transfection rates with low methodology-induced cytotoxic side effects are essential to attain the required effect on targeted cells when exogenous DNA is transfected. Different approaches and ...
Gold Nanoparticle Mediated Laser Transfection for Efficient siRNA Mediated Gene Knock Down

Heinemann, Dag; Schomaker, Markus; Kalies, Stefan; Schieck, Maximilian; Carlson, Regina; Murua Escobar, Hugo; Ripken, Tammo; Meyer, Heiko; Heisterkamp, Alexander (San Francisco, California, US : PLOS, 2013)

Laser based transfection methods have proven to be an efficient and gentle alternative to established molecule delivery methods like lipofection or electroporation. Among the laser based methods, gold nanoparticle mediated ...
Investigation of biophysical mechanisms in gold nanoparticle mediated laser manipulation of cells using a multimodal holographic and fluorescence imaging setup

Kalies, Stefan; Antonopoulos, Georgios C.; Rakoski, Mirko S.; Heinemann, Dag; Schomaker, Markus; Ripken, Tammo; Meyer, Heiko (San Francisco, CA : Public Library of Science (PLoS), 2015)

Laser based cell manipulation has proven to be a versatile tool in biomedical applications. In this context, combining weakly focused laser pulses and nanostructures, e.g. gold nanoparticles, promises to be useful for high ...
Polyethylenimine-mediated gene delivery into human bone marrow mesenchymal stem cells from patients

Wang, Weiwei; Li, Wenzhong; Ou, Lailiang; Flick, Eva; Mark, Peter; Nesselmann, Catharina; Lux, Cornelia A.; Gatzen, Hanz-Heinrich; Kaminski, Alexander; Liebold, Andreas; Lützow, Karola; Lendlein, Andreas; Li, Ren-Ke; Steinhoff, Gustav; Ma, Nan (Chichester : Wiley-Blackwell Publishing Ltd., 2011)

Transplantation of mesenchymal stem cells (MSCs) derived from adult bone marrow has been proposed as a potential therapeutic approach for post-infarction left ventricular (LV) dysfunction. However, age-related functional ...

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Browsing by Subject "genetic transfection"

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