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Characterisation of a Recombinant Patchoulol Synthase Variant for Biocatalytic Production of Terpenes

Frister, Thore; Hartwig, Steffen; Alemdar, Semra; Schnatz, Katharina; Thöns, Laura; Scheper, Thomas; Beutel, Sascha (New York City, NY : Springer Humana Press Inc., 2015)

The patchoulol synthase (PTS) is a multi-product sesquiterpene synthases which is the central enzyme for biosynthesis of patchouli essential oil in the patchouli plant. Sesquiterpene synthases catalyse the formation of ...
Heterologous Expression, Purification, and Biochemical Characterization of α-Humulene Synthase from Zingiber zerumbet Smith

Alemdar, Semra; Hartwig, Steffen; Frister, Thore; König, Jan Christoph; Scheper, Thomas; Beutel, Sascha (New York City, NY : Springer Humana Press Inc., 2016)

The α-humulene synthase from Zingiber zerumbet Smith was expressed as a polyhistidine-tagged protein in an E. coli BL21(DE3) strain. Induction time and inductor (isopropyl-β-D-thiogalactopyranoside) concentration were ...
Optimization of factors influencing enzyme activity and product selectivity and the role of proton transfer in the catalytic mechanism of patchoulol synthase

Ekramzadeh, Kimia; Brämer, Chantal; Frister, Thore; Fohrer, Jörg; Kirschning, Andreas; Scheper, Thomas; Beutel, Sascha (Malden, MA : Wiley, 2020)

The patchoulol synthase (PTS) from Pogostemon cablin is a versatile sesquiterpene synthase and produces more than 20 valuable sesquiterpenes by conversion of the natural substrate farnesyl pyrophosphate (FPP). PTS has the ...
SUMO-fusion, purification, and characterization of a (+)-zizaene synthase from Chrysopogon zizanioides

Hartwig, Steffen; Frister, Thore; Alemdar, Semra; Li, Zhaopeng; Scheper, Thomas; Beutel, Sascha (Cambridge, MA : Elsevier Academic Press, 2015)

An uncharacterized plant cDNA coding for a polypeptide presumably having sesquiterpene synthase activity, was expressed in soluble and active form. Two expression strategies were evaluated in Escherichia coli. The enzyme ...

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Browsing by Author "2c225c5d-ad0d-4f62-90e9-dc9a3a51c63c"

Browsing by Author "2c225c5d-ad0d-4f62-90e9-dc9a3a51c63c"

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