Biofunctional quantum dots as fluorescence probe for cell-specific targeting

Originalpublikation

Ag, D.; Bongartz, R.; Dogan, L.E.; Seleci, M.; Walter, J.-G.; et al.: Biofunctional quantum dots as fluorescence probe for cell-specific targeting. In: Colloids and Surfaces B: Biointerfaces 114 (2014), S. 96-103. DOI: https://doi.org/10.1016/j.colsurfb.2013.09.033

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Zusammenfassung:
We describe here the synthesis, characterization, bioconjugation, and application of water-soluble thioglycolic acid TGA-capped CdTe/CdS quantum dots (TGA-QDs) for targeted cellular imaging. Anti-human epidermal growth factor receptor 2 (HER2) antibodies were conjugated to TGA-QDs to target HER2-overexpressing cancer cells. TGA-QDs and TGA-QDs/anti-HER2 bioconjugates were characterized by fluorescence and UV-Vis spectroscopy, X-ray diffraction (XRD), hydrodynamic sizing, electron microscopy, and gel electrophoresis. TGA-QDs and TGA-QDs/anti-HER2 were incubated with cells to examine cytotoxicity, targeting efficiency, and cellular localization. The cytotoxicity of particles was measured using an MTT assay and the no observable adverse effect concentration (NOAEC), 50% inhibitory concentration (IC50), and total lethal concentration (TLC) were calculated. To evaluate localization and targeting efficiency of TGA-QDs with or without antibodies, fluorescence microscopy and flow cytometry were performed. Our results indicate that antibody-conjugated TGA-QDs are well-suited for targeted cellular imaging studies.
Lizenzbestimmungen: CC BY-NC-ND 3.0 Unported - https://creativecommons.org/licenses/by-nc-nd/3.0/
Publikationstyp: Article
Publikationsstatus: publishedVersion
Erstveröffentlichung: 2014
Schlagwörter (englisch): Anti-HER2, Bioconjugation, Cell specific targeting, Imaging, Quantum dots, Electrophoresis, Fluorescence, Fluorescence microscopy, Imaging techniques, Semiconductor quantum dots, Ultraviolet visible spectroscopy, X ray diffraction, Anti-HER2, Bio-conjugation, Cellular localization, Epidermal growth factor receptor 2, Fluorescence probes, Gel electrophoresis, Inhibitory concentration, Lethal concentration, Antibodies, 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, cadmium, epidermal growth factor receptor 2, epidermal growth factor receptor 2 antibody, quantum dot, receptor antibody, sulfur, tellurium, thioglycolic acid, unclassified drug, animal cell, cancer cell, cancer diagnosis, cell assay, cell labeling, cell specificity, cell surface, cellular distribution, controlled study, drug concentration, drug conjugation, drug cytotoxicity, drug design, drug solubility, drug synthesis, electron microscopy, flow cytometry, fluorescence imaging, fluorescence microscopy, human cell, hydrodynamics, internalization, lung cancer, mouse, no observable adverse effect concentration, nonhuman, priority journal, protein expression, target cell, total lethal concentration, ultraviolet spectroscopy, X ray diffraction, Anti-HER2, Cell specific targeting, Cadmium Compounds, Cell Death, Cell Membrane, Cell Survival, Cells, Fluorescent Dyes, Light, Mice, Microscopy, Fluorescence, NIH 3T3 Cells, Receptor, erbB-2, Reproducibility of Results, Tellurium, Thioglycolates, X-Ray Diffraction
Fachliche Zuordnung (DDC): 570 | Biowissenschaften, Biologie

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