Lesker, Till; Maiss, Edgar: In planta Protein Interactions of Three Alphacryptoviruses and Three Betacryptoviruses from White Clover, Red Clover and Dill by Bimolecular Fluorescence Complementation Analysis. In: Viruses 5 (2013), Nr. 10, S. 2512-2530. DOI:
http://dx.doi.org/10.3390/v5102512
Zusammenfassung: |
Plant-infecting viruses of the genera Alpha- and Betacryptovirus within the family Partitiviridae cause no visible effects on their hosts and are only transmitted by cell division and through gametes. The bipartite dsRNA genome is encoding a RNA-dependent RNA polymerase (RdRp) and a coat protein (CP). Aside from sequence and structural analysis, the investigation of protein interactions is another step towards virus characterization. Therefore, ORFs of two type members White Clover Cryptic Virus 1 and 2 (WCCV-1 and WCCV-2), as well as the related viruses from Red Clover and Dill were introduced into a bimolecular fluorescence complementation assay. We showed CP-CP dimerization for all tested viruses with localization for alphacryptoviruses at the nuclear membrane and for betacryptoviruses close to cell walls within the cytoplasm. For CPs of WCCV-1 and WCCV-2, deletion mutants were created to determine internal interaction sites. Moreover, RdRp self-interaction was found for all viruses, whereas CP-RdRp interactions were only detectable for the alphacryptoviruses. An intra-genus test of CPs was successful in various virus combinations, whereas an inter-genus interaction of WCCV-1CP and WCCV-2CP was absent. This is the first report of in vivo protein interactions of members in the family Partitiviridae, indicating distinct features of the alpha- and betacryptoviruses.
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Lizenzbestimmungen: |
CC BY 3.0 Unported - http://creativecommons.org/licenses/by/3.0/
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Publikationstyp: |
Article |
Publikationsstatus: |
publishedVersion |
Erstveröffentlichung: |
2013-10-09 |
Schlagwörter (deutsch): |
Partitiviridae, Alphacryptovirus, Betacryptovirus, Protein-Wechselwirkung, Bimolekulare Fluoreszenzkomplementation, BiFC, Trifolium repens, Trifolium pratense, Anethum graveolens
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Schlagwörter (englisch): |
Partitiviridae, Alphacryptovirus, Betacryptovirus, protein interaction, Bimolecular fluorescence complementation, BiFC, White Clover, Red Clover, Dill
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Fachliche Zuordnung (DDC): |
570 | Biowissenschaften, Biologie, 580 | Pflanzen (Botanik)
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Kontrollierte Schlagwörter: |
Pflanzenviren, Protein-Protein-Wechselwirkung, Weißklee, Rotklee, Dill
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