Zusammenfassung: | |
Background: Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.Methods: We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.Results: The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.Conclusions: VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.
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Lizenzbestimmungen: | CC BY 2.0 Unported - https://creativecommons.org/licenses/by/2.0/ |
Publikationstyp: | Article |
Publikationsstatus: | publishedVersion |
Erstveröffentlichung: | 2013 |
Schlagwörter (englisch): | Bionanoparticles, Dengue envelope domain III, Hepatitis B surface antigen, Pichia pastoris, Virus-like particle, Bionanoparticles, Envelope domains, Hepatitis B surface antigen, Pichia Pastoris, Virus-like particles, Antigens, Diagnosis, Medical applications, Viruses, antigen, hepatitis B surface antigen, nanoparticle, s antigen, unclassified drug, virus envelope protein, cell extract, hybrid protein, nanoparticle, S envelope protein, hepatitis B virus, virus antigen, virus protein, article, cell lysate, Dengue virus, gene dosage, gene expression, molecular cloning, nonhuman, Pichia pastoris, protein domain, virus like agent, animal, chemistry, Chlorocebus aethiops, dengue, Dengue virus, isolation and purification, metabolism, physiology, Pichia, protein tertiary structure, species difference, ultrastructure, Vero cell line, virion, virology, Dengue virus, Hepatitis B virus, Pichia pastoris, Animals, Antigens, Viral, Cell Extracts, Cercopithecus aethiops, Dengue, Dengue Virus, Nanoparticles, Pichia, Protein Structure, Tertiary, Recombinant Fusion Proteins, Species Specificity, Vero Cells, Viral Envelope Proteins, Viral Proteins, Virion |
Fachliche Zuordnung (DDC): | 570 | Biowissenschaften, Biologie, 610 | Medizin, Gesundheit |
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