dc.identifier.uri |
http://dx.doi.org/10.15488/4195 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/4229 |
|
dc.contributor.author |
Saklayen, Nabiha
|
|
dc.contributor.author |
Kalies, Stefan
|
|
dc.contributor.author |
Madrid, Marinna
|
|
dc.contributor.author |
Nuzzo, Valeria
|
|
dc.contributor.author |
Huber, Marinus
|
|
dc.contributor.author |
Shen, Weilu
|
|
dc.contributor.author |
Sinanan-Singh, Jasmine
|
|
dc.contributor.author |
Heinemann, Dag
|
|
dc.contributor.author |
Heisterkamp, Alexander
|
|
dc.contributor.author |
Mazur, Eric
|
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dc.date.accessioned |
2018-12-17T13:14:07Z |
|
dc.date.available |
2018-12-17T13:14:07Z |
|
dc.date.issued |
2017 |
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dc.identifier.citation |
Saklayen, N.; Kalies, S.; Madrid, M.; Nuzzo, V.; Huber, M. et al.: Analysis of poration-induced changes in cells from laser-activated plasmonic substrates. In: Biomedical Optics Express 8 (2017), Nr. 10, S. 4756-4771. DOI: https://doi.org/10.1364/BOE.8.004756 |
|
dc.description.abstract |
Laser-exposed plasmonic substrates permeabilize the plasma membrane of cells when in close contact to deliver cell-impermeable cargo. While studies have determined the cargo delivery efficiency and viability of laser-exposed plasmonic substrates, morphological changes in a cell have not been quantified. We porated myoblast C2C12 cells on a plasmonic pyramid array using a 532-nm laser with 850-ps pulse length and time-lapse fluorescence imaging to quantify cellular changes. We obtain a poration efficiency of 80%, viability of 90%, and a pore radius of 20 nm. We quantified area changes in the plasma membrane attached to the substrate (10% decrease), nucleus (5 - 10% decrease), and cytoplasm (5 - 10% decrease) over 1 h after laser treatment. Cytoskeleton fibers show a change of 50% in the alignment, or coherency, of fibers, which stabilizes after 10 mins. We investigate structural and morphological changes due to the poration process to enable the safe development of this technique for therapeutic applications. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Washington, DC : Optical Society of America |
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dc.relation.ispartofseries |
Biomedical Optics Express 8 (2017), Nr. 10 |
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dc.rights |
OSA Open Access License |
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dc.rights.uri |
https://www.osapublishing.org/submit/review/open-access-policy-statement.cfm |
|
dc.subject |
(000.1430) biology and medicine |
eng |
dc.subject |
(190.4870) photothermal effects |
eng |
dc.subject |
(350.4855) optical tweezers or optical manipulation |
eng |
dc.subject |
Cell membranes |
eng |
dc.subject |
Cells |
eng |
dc.subject |
Efficiency |
eng |
dc.subject |
Morphology |
eng |
dc.subject |
Optical tweezers |
eng |
dc.subject |
Plasmons |
eng |
dc.subject |
Substrates |
eng |
dc.subject |
Biology and medicine |
eng |
dc.subject |
Cellular changes |
eng |
dc.subject |
Fluorescence imaging |
eng |
dc.subject |
Laser treatment |
eng |
dc.subject |
Morphological changes |
eng |
dc.subject |
Optical manipulation |
eng |
dc.subject |
Photothermal effects |
eng |
dc.subject |
Therapeutic Application |
eng |
dc.subject |
Cytology |
eng |
dc.subject.ddc |
530 | Physik
|
ger |
dc.title |
Analysis of poration-induced changes in cells from laser-activated plasmonic substrates |
eng |
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.issn |
21567085 |
|
dc.relation.doi |
https://doi.org/10.1364/BOE.8.004756 |
|
dc.bibliographicCitation.issue |
10 |
|
dc.bibliographicCitation.volume |
8 |
|
dc.bibliographicCitation.firstPage |
4756 |
|
dc.bibliographicCitation.lastPage |
4771 |
|
dc.description.version |
publishedVersion |
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tib.accessRights |
frei zug�nglich |
|