dc.identifier.uri |
http://dx.doi.org/10.15488/17128 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/17256 |
|
dc.contributor.author |
Heinemann, Henrike
|
|
dc.contributor.author |
Zhang, Haili
|
|
dc.contributor.author |
Cox, Russell J.
|
|
dc.date.accessioned |
2024-04-18T06:09:21Z |
|
dc.date.available |
2024-04-18T06:09:21Z |
|
dc.date.issued |
2023 |
|
dc.identifier.citation |
Heinemann, H.; Zhang, H.; Cox, R.J.: Reductive Release from a Hybrid PKS-NRPS during the Biosynthesis of Pyrichalasin H. In: Chemistry – A European Journal 30 (2024), Nr. 4, e202302590. DOI: https://doi.org/10.1002/chem.202302590 |
|
dc.description.abstract |
Three central steps during the biosynthesis of cytochalasan precursors, including reductive release, Knoevenagel cyclisation and Diels Alder cyclisation are not yet understood at a detailed molecular level. In this work we investigated the reductive release step catalysed by a hybrid polyketide synthase non-ribosomal peptide synthetase (PKS-NRPS) from the pyrichalasin H pathway. Synthetic thiolesters were used as substrate mimics for in vitro studies with the isolated reduction (R) and holo-thiolation (T) domains of the PKS-NRPS hybrid PyiS. These assays demonstrate that the PyiS R-domain mainly catalyses an NADPH-dependent reductive release of an aldehyde intermediate that quickly undergoes spontaneous Knoevenagel cyclisation. The R-domain can only process substrates that are covalently bound to the phosphopantetheine thiol of the upstream T-domain, but it shows little selectivity for the polyketide. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Weinheim : Wiley-VCH |
|
dc.relation.ispartofseries |
Chemistry – A European Journal 30 (2024), Nr. 4 |
|
dc.rights |
CC BY-NC 4.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc/4.0 |
|
dc.subject |
cytochalasan |
eng |
dc.subject |
non-ribosomal peptide |
eng |
dc.subject |
polyketide |
eng |
dc.subject |
reductive release |
eng |
dc.subject |
SDR |
eng |
dc.subject.ddc |
540 | Chemie
|
|
dc.subject.ddc |
660 | Technische Chemie
|
|
dc.title |
Reductive Release from a Hybrid PKS-NRPS during the Biosynthesis of Pyrichalasin H |
eng |
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.essn |
1521-3765 |
|
dc.relation.issn |
0947-6539 |
|
dc.relation.doi |
https://doi.org/10.1002/chem.202302590 |
|
dc.bibliographicCitation.issue |
4 |
|
dc.bibliographicCitation.volume |
30 |
|
dc.bibliographicCitation.date |
2024 |
|
dc.bibliographicCitation.firstPage |
e202302590 |
|
dc.description.version |
publishedVersion |
eng |
tib.accessRights |
frei zug�nglich |
|
dc.bibliographicCitation.articleNumber |
e202302590 |
|