dc.identifier.uri |
http://dx.doi.org/10.15488/15954 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/16080 |
|
dc.contributor.author |
Tian, Dong-Song
|
|
dc.contributor.author |
Kuhnert, Eric
|
|
dc.contributor.author |
Ouazzani, Jamal
|
|
dc.contributor.author |
Wibberg, Daniel
|
|
dc.contributor.author |
Kalinowski, Jörn
|
|
dc.contributor.author |
Cox, Russell J.
|
|
dc.date.accessioned |
2024-01-18T09:11:34Z |
|
dc.date.available |
2024-01-18T09:11:34Z |
|
dc.date.issued |
2020 |
|
dc.identifier.citation |
Tian, D.-S.; Kuhnert, E.; Ouazzani, J.; Wibberg, D.; Kalinowski, J. et al.: The sporothriolides. A new biosynthetic family of fungal secondary metabolites. In: Chemical Science 11 (2020), Nr. 46, S. 12477-12484. DOI: https://doi.org/10.1039/d0sc04886k |
|
dc.description.abstract |
The biosynthetic gene cluster of the antifungal metabolite sporothriolide 1 was identified from three producing ascomycetes: Hypomontagnella monticulosa MUCL 546, H. spongiphila CLL 205 and H. submonticulosa DAOMC 242471. A transformation protocol was established, and genes encoding a fatty acid synthase subunit and a citrate synthase were simultaneously knocked out which led to loss of sporothriolide and sporochartine production. In vitro reactions showed that the sporochartines are derived from non-enzymatic Diels-Alder cycloaddition of 1 and trienylfuranol A 7 during the fermentation and extraction process. Heterologous expression of the spo genes in Aspergillus oryzae then led to the production of intermediates and shunts and delineation of a new fungal biosynthetic pathway originating in fatty acid biosynthesis. Finally, a hydrolase was revealed by in vitro studies likely contributing towards self-resistance of the producer organism. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Cambridge : RSC |
|
dc.relation.ispartofseries |
Chemical Science 11 (2020), Nr. 46 |
|
dc.rights |
CC BY-NC 3.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc/3.0 |
|
dc.subject |
Aspergillus |
eng |
dc.subject |
Biochemistry |
eng |
dc.subject |
Biosynthesis |
eng |
dc.subject |
Cycloaddition |
eng |
dc.subject |
Genes |
eng |
dc.subject.ddc |
540 | Chemie
|
|
dc.title |
The sporothriolides. A new biosynthetic family of fungal secondary metabolites |
eng |
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.essn |
2041-6539 |
|
dc.relation.issn |
2041-6520 |
|
dc.relation.doi |
https://doi.org/10.1039/d0sc04886k |
|
dc.bibliographicCitation.issue |
46 |
|
dc.bibliographicCitation.volume |
11 |
|
dc.bibliographicCitation.firstPage |
12477 |
|
dc.bibliographicCitation.lastPage |
12484 |
|
dc.description.version |
publishedVersion |
|
tib.accessRights |
frei zug�nglich |
|