Heinemann, H.; Zhang, H.; Cox, R.J.: Reductive Release from a Hybrid PKS-NRPS during the Biosynthesis of Pyrichalasin H. In: Chemistry – A European Journal 30 (2024), Nr. 4, e202302590. DOI: https://doi.org/10.1002/chem.202302590
Abstract: | |
Three central steps during the biosynthesis of cytochalasan precursors, including reductive release, Knoevenagel cyclisation and Diels Alder cyclisation are not yet understood at a detailed molecular level. In this work we investigated the reductive release step catalysed by a hybrid polyketide synthase non-ribosomal peptide synthetase (PKS-NRPS) from the pyrichalasin H pathway. Synthetic thiolesters were used as substrate mimics for in vitro studies with the isolated reduction (R) and holo-thiolation (T) domains of the PKS-NRPS hybrid PyiS. These assays demonstrate that the PyiS R-domain mainly catalyses an NADPH-dependent reductive release of an aldehyde intermediate that quickly undergoes spontaneous Knoevenagel cyclisation. The R-domain can only process substrates that are covalently bound to the phosphopantetheine thiol of the upstream T-domain, but it shows little selectivity for the polyketide. | |
License of this version: | CC BY-NC 4.0 Unported |
Document Type: | Article |
Publishing status: | publishedVersion |
Issue Date: | 2023 |
Appears in Collections: | Naturwissenschaftliche Fakultät |
pos. | country | downloads | ||
---|---|---|---|---|
total | perc. | |||
1 | China | 1 | 100.00% |
Hinweis
Zur Erhebung der Downloadstatistiken kommen entsprechend dem „COUNTER Code of Practice for e-Resources“ international anerkannte Regeln und Normen zur Anwendung. COUNTER ist eine internationale Non-Profit-Organisation, in der Bibliotheksverbände, Datenbankanbieter und Verlage gemeinsam an Standards zur Erhebung, Speicherung und Verarbeitung von Nutzungsdaten elektronischer Ressourcen arbeiten, welche so Objektivität und Vergleichbarkeit gewährleisten sollen. Es werden hierbei ausschließlich Zugriffe auf die entsprechenden Volltexte ausgewertet, keine Aufrufe der Website an sich.