Increasing transport activities in cities are a substantial driver for congestion and pollution, influencing urban populations’ health and quality of life. These effects are consequences of ongoing urbanization in combination with rising individual demand for mobility, goods, and services. With the goal of increased environmental sustainability in urban areas, city authorities and politics aim for reduced traffic and minimized transport emissions.To support more efficient and sustainable urban transport, this cumulative dissertation focuses on alternative transport concepts. For this purpose, scientific methods and models of the interdisciplinary information systems domain combined with elements of operations research, transportation, and logistics are developed and investigated in multiple research contributions. Different transport concepts are examined in terms of optimization and acceptance to provide decision support for relevant stakeholders. In more detail, the overarching topic of urban transport in this dissertation is divided into the complexes urban mobility (part A) in terms of passenger transport and urban logistics (part B) with a focus on the delivery of goods and services. Within part A, approaches to carsharing optimization are presented at various planning levels. Furthermore, the user acceptance of ridepooling is investigated. Part B outlines several optimization models for alternative urban parcel and e-grocery delivery concepts by proposing different network structures and transport vehicles. Conducted surveys on intentional use of urban logistics concepts give valuable hints to providers and decision makers. The introduced approaches with their corresponding results provide target-oriented support to facilitate decision making based on quantitative data. Due to the continuous growth of urban transport, the relevance of decision support in this regard, but also the understanding of the key drivers for people to use certain services will further increase in the future. By providing decision support for urban mobility as well as urban logistics concepts, this dissertation contributes to enhanced economic, social, and environmental sustainability in urban areas.