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Browsing by Subject "Cell culture"

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3D-printed individual labware in biosciences by rapid prototyping: In vitro biocompatibility and applications for eukaryotic cell cultures

Lücking, Tim H.; Sambale, Franziska; Schnaars, Birte; Bulnes-Abundis, David; Beutel, Sascha; Scheper, Thomas (Weinheim : Wiley-VCH Verlag, 2014)

Three-dimensional (3D) printing techniques are continuously evolving, thus their application fields are also growing very fast. The applications discussed here highlight the use of rapid prototyping in a dedicated biotechnology ...
Characterization of a customized 3D-printed cell culture system using clear, translucent acrylate that enables optical online monitoring

Siller, Ina Gerhild; Enders, Anton; Gellermann, Pia; Winkler, Steffen; Lavrentieva, Antonina; Scheper, Thomas; Bahnemann, Janina (Bristol : Institute of Physics Publishing, 2020)

Cells are very sensitive to their direct environment - they place high demands, for example, on ambient culture medium, adjacent cell types, and the properties of surrounding material parts. As a result, mechanical and ...
Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: Efficiency and cytotoxicity

Durán, Maria Carolina; Willenbrock, Saskia; Barchanski, Annette; Müller, Jessika-M.V.; Maiolini, Arianna; Soller, Jan T.; Barcikowski, Stephan; Nolte, Ingo; Feige, Karsten; Murua Escobar, Hugo (London : Biomed Central, 2011)

Background: Reproducibly high transfection rates with low methodology-induced cytotoxic side effects are essential to attain the required effect on targeted cells when exogenous DNA is transfected. Different approaches and ...
Comparison of six different silicones in vitro for application as glaucoma drainage device

Windhövel, Claudia; Harder, Lisa; Bach, Jan-Peter; Teske, Michael; Grabow, Niels; Eickner, Thomas; Hinze, Ulf; Chichkov, Boris; Nolte, Ingo (Basel : MDPI AG, 2018)

Silicones are widely used in medical applications. In ophthalmology, glaucoma drainage devices are utilized if conservative therapies are not applicable or have failed. Long-term success of these devices is limited by ...
Customizable 3D-printed (Co-)cultivation systems for in vitro study of angiogenesis

Siller, Ina G.; Epping, Niklas-Maximilian; Lavrentieva, Antonina; Scheper, Thomas; Bahnemann, Janina (Basel : MDPI AG, 2020)

Due to the ever-increasing resolution of 3D printing technology, additive manufacturing is now even used to produce complex devices for laboratory applications. Personalized experimental devices or entire cultivation systems ...
Effects of degradable Mg-Ca alloys on dendritic cell function

Feser, K.; Kietzmann, M.; Bäumer, W.; Krause, C.; Bach, F.W. (London : SAGE Publications Ltd., 2011)

Degradable magnesium alloys are new materials for implants used in orthopedic and trauma surgery. The aim of this study was to investigate the influence of degradable magnesium alloys on the function of dendritic cells ...
Evaluation of the biological tolerability of the starch-based medical device 4DryField® PH in vitro and in vivo a rat model

Poehnert, Daniel; Abbas, Mahmoud; Maegel, Lavinia; Sambale, Franziska; Lavrentieva, Antonina; Kreipe, Hans-Heinrich; Klempnauer, Jürgen; Winny, Markus (London : SAGE Publications Ltd., 2015)

Purpose To evaluate in vitro cytotoxicity/biocompatibility as well as in vivo tolerability of the novel polysaccharide 4DryField® PH, certified for haemostasis and adhesion prevention. Methods In vitro cytotoxicity/viability ...
Femtosecond laser fabricated spike structures for selective control of cellular behavior

Schlie, Sabrina; Fadeeva, Elena; Koch, Jürgen; Ngezahayo, Anaclet; Chichkov, Boris (London : SAGE Publications Ltd., 2010)

In this study we investigate the potential of femtosecond laser generated micrometer sized spike structures as functional surfaces for selective cell controlling. The spike dimensions as well as the average spike to spike ...
Impact of source and manufacturing of collagen matrices on fibroblast cell growth and platelet aggregation

Böhm, Stefanie; Strauß, Christine; Stoiber, Stefan; Kasper, Cornelia; Charwat, Verena (Basel : MDPI AG, 2017)

Collagen is a main component of the extracellular matrix. It is often used in medical applications to support tissue regeneration, hemostasis, or wound healing. Due to different sources of collagen, the properties and ...
Longitudinal claudin gene expression analyses in canine mammary tissues and thereof derived primary cultures and cell lines

Hammer, Susanne C.; Becker, Annegret; Rateitschak, Katja; Mohr, Annika; Ripoli, Florenza Lüder; Hennecke, Silvia; Junginger, Johannes; Hewicker-Trautwein, Marion; Brenig, Bertram; Ngezahayo, Anaclet; Nolte, Ingo; Escobar, Hugo Murua (Basel : MDPI AG, 2016)

Human and canine mammary tumours show partial claudin expression deregulations. Further, claudins have been used for directed therapeutic approaches. However, the development of claudin targeting approaches requires stable ...
Mesoporous silica coatings for controlled release of the antibiotic ciprofloxacin from implants

Ehlert, Nina; Badar, Muhammad; Christel, Anne; Lohmeier, Sven Jare; Luessenhop, Tammo; Stieve, Martin; Lenarz, Thomas; Mueller, Peter Paul; Behrens, Peter (Cambridge : Royal Society of Chemistry, 2011)

To generate bioactive coatings for medical implants, a novel procedure has been developed using a coating of mesoporous silica for controlled drug delivery. Plain glass slides were used as substrates. The mesoporous coatings ...
Nanostructured bifunctional hydrogels as potential instructing platform for hematopoietic stem cell differentiation

Kratzer, Domenic; Ludwig-Husemann, Anita; Junges, Katharina; Geckle, Udo; Lee-Thedieck, Cornelia (Lausanne : Frontiers Media S.A., 2019)

Hematopoietic stem cells (HSCs) are blood forming cells which possess the ability to differentiate into all types of blood cells. T cells are one important cell type HSCs can differentiate into, via corresponding progenitor ...
The osteogenic and tenogenic differentiation potential of C3H10T1/2 (mesenchymal stem cell model) cultured on PCL/PLA electrospun scaffolds in the absence of specific differentiation medium

Baudequin, Timothée; Gaut, Ludovic; Mueller, Marc; Huepkes, Angela; Glasmacher, Birgit; Duprez, Delphine; Bedoui, Fahmi; Legallais, Cécile (Basel : MDPI AG, 2017)

The differentiation potential of mesenchymal stem cells (MSC) has been extensively tested on electrospun scaffolds. However, this potential is often assessed with lineage-specific medium, making it difficult to interpret ...
Osteointegration of porous poly-ε-caprolactone-coated and previtalised magnesium implants in critically sized calvarial bone defects in the mouse model

Grau, Michael; Seiler, Christian; Roland, Laura; Matena, Julia; Windhövel, Claudia; Teske, Michael; Escobar, Hugo Murua; Lüpke, Matthias; Seifert, Hermann; Gellrich, Nils-Claudius; Haferkamp, Heinz; Nolte, Ingo (Basel : MDPI AG, 2017)

Metallic biomaterials are widely used in maxillofacial surgery. While titanium is presumed to be the gold standard, magnesium-based implants are a current topic of interest and investigation due to their biocompatible, ...
Phenotypic drug screening in a human fibrosis model identified a novel class of antifibrotic therapeutics

Gerckens, Michael; Schorpp, Kenji; Pelizza, Francesco; Wögrath, Melanie; Reichau, Kora; Ma, Huilong; Dworsky, Armando-Marco; Sengupta, Arunima; Stoleriu, Mircea Gabriel; Heinzelmann, Katharina; Merl-Pham, Juliane; Irmler, Martin; Alsafadi, Hani N.; Trenkenschuh, Eduard; Sarnova, Lenka; Jirouskova, Marketa; Frieß, Wolfgang; Hauck, Stefanie M.; Beckers, Johannes; Kneidinger, Nikolaus; Behr, Jürgen; Hilgendorff, Anne; Hadian, Kamyar; Lindner, Michael; Königshoff, Melanie; Eickelberg, Oliver; Gregor, Martin; Plettenburg, Oliver; Yildirim, Ali Önder; Burgstaller, Gerald (Washington, DC [u.a.] : Assoc., 2021)

Fibrogenic processes instigate fatal chronic diseases leading to organ failure and death. Underlying biological processes involve induced massive deposition of extracellular matrix (ECM) by aberrant fibroblasts. We subjected ...
Real-time live-cell imaging technology enables high-throughput screening to verify in vitro biocompatibility of 3D printed materials

Siller, Ina G.; Enders, Anton; Steinwedel, Tobias; Epping, Niklas-Maximilian; Kirsch, Marline; Lavrentieva, Antonina; Scheper, Thomas; Bahnemann, Janina (Basel : MDPI AG, 2019)

With growing advances in three-dimensional (3D) printing technology, the availability and diversity of printing materials has rapidly increased over the last years. 3D printing has quickly become a useful tool for biomedical ...
Sensor integration into microfluidic systems: trends and challenges

Buttkewitz, Marc A.; Heuer, Christopher; Bahnemann, Janina (Amsterdam [u.a.] : Elsevier Science, 2023)

The combination of sensors and microfluidics has become a promising approach for detecting a wide variety of targets relevant in biotechnology. Thanks to recent advances in the manufacturing of microfluidic systems, ...
Synthesis and antiproliferative activity of new tonantzitlolone-derived diterpene derivatives

Busch, Torsten; Dräger, Gerald; Kunst, Eike; Benson, Hannah; Sasse, Florenz; Siems, Karsten; Kirschning, Andreas (Cambridge : Royal Society of Chemistry, 2016)

The synthesis of the diterpene (+)-tonantzitlolone A and a series of derivatives is reported. The study includes the determination of their antiproliferative activities against selected cancer cell lines. © The Royal Society ...
Tumor homing and penetrating peptide-conjugated niosomes as multi-drug carriers for tumor-targeted drug delivery

Ag Seleci, Didem; Seleci, Muharre,; Stahl, Frank; Scheper, Thomas (Cambridge : Royal Society of Chemistry, 2017)

The development of nanoscale drug delivery systems, which can mediate efficient tumor targeting together with high cellular internalization, is crucial for glioma treatment. The combination of therapeutic agents in ...

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