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Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures

Vanz, Ana Leticia; Nimtz, Manfred; Rinas, Ursula (London : BioMed Central Ltd., 2014)

Background: Pichia pastoris is a popular yeast preferably employed for secretory protein production. Secretion is not always efficient and endoplasmic retention of proteins with aberrant folding properties, or when produced ...
Dengue-specific subviral nanoparticles: Design, creation and characterization

Khetarpal, Niyati; Poddar, Ankur; Nemani, Satish K.; Dhar, Nisha; Patil, Aravind; Negi, Priyanka; Perween, Ashiya; Viswanathan, Ramaswamy; Lünsdorf, Heinrich; Tyagi, Poornima; Raut, Rajendra; Arora, Upsana; Jain, Swatantra K.; Rinas, Ursula; Swaminathan, Sathyamangalam; Khanna, Navin (London : BioMed Central Ltd., 2013)

Background: Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing ...
Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: Catabolic adaptation, stress responses, and autophagic processes

Vanz, Ana Leticia; Lünsdorf, Heinrich; Adnan, Ahmad; Nimtz, Manfred; Gurramkonda, Chandrasekhar; Khanna, Navin; Rinas, Ursula (London : BioMed Central Ltd., 2012)

Background: Pichia pastoris is an established eukaryotic host for the production of recombinant proteins. Most often, protein production is under the control of the strong methanol-inducible aox1 promoter. However, detailed ...
Virus-like particle production with yeast: Ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen

Lünsdorf, Heinrich; Gurramkonda, Chandrasekhar; Adnan, Ahmad; Khanna, Navin; Rinas, Ursula (London : BioMed Central Ltd., 2011)

Background: A protective immune response against Hepatitis B infection can be obtained through the administration of a single viral polypeptide, the Hepatitis B surface antigen (HBsAg). Thus, the Hepatitis B vaccine is ...

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