dc.identifier.uri |
http://dx.doi.org/10.15488/8802 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/8855 |
|
dc.contributor.author |
Aguilar Cascante, Francisco José
|
|
dc.contributor.author |
Scheper, Thomas
|
|
dc.contributor.author |
Beutel, Sascha
|
|
dc.date.accessioned |
2019-12-11T16:12:42Z |
|
dc.date.available |
2019-12-11T16:12:42Z |
|
dc.date.issued |
2019 |
|
dc.identifier.citation |
Aguilar, F.; Scheper, T.; Beutel, S.: Improved production and in situ recovery of sesquiterpene (+)-zizaene from metabolically-engineered E. Coli. In: Molecules 24 (2019), Nr. 18, 3356. DOI: https://doi.org/10.3390/molecules24183356 |
|
dc.description.abstract |
The sesquiterpene (+)-zizaene is the direct precursor of khusimol, the main fragrant compound of the vetiver essential oil from Chrysopogon zizanioides and used in nearly 20% of men’s fine perfumery. The biotechnological production of such fragrant sesquiterpenes is a promising alternative towards sustainability; nevertheless, product recovery from fermentation is one of the main constraints. In an effort to improve the (+)-zizaene recovery from a metabolically-engineered Escherichia coli, we developed an integrated bioprocess by coupling fermentation and (+)-zizaene recovery using adsorber extractants. Initially, (+)-zizaene volatilization was confirmed from cultivations with no extractants but application of liquid–liquid phase partitioning cultivation (LLPPC) improved (+)-zizaene recovery nearly 4-fold. Furthermore, solid–liquid phase partitioning cultivation (SLPPC) was evaluated by screening polymeric adsorbers, where Diaion HP20 reached the highest recovery. Bioprocess was scaled up to 2 L bioreactors and in situ recovery configurations integrated to fermentation were evaluated. External recovery configuration was performed with an expanded bed adsorption column and improved (+)-zizaene titers 2.5-fold higher than LLPPC. Moreover, internal recovery configuration (IRC) further enhanced the (+)-zizaene titers 2.2-fold, whereas adsorption velocity was determined as critical parameter for recovery efficiency. Consequently, IRC improved the (+)-zizaene titer 8.4-fold and productivity 3-fold from our last report, achieving a (+)-zizaene titer of 211.13 mg L−1 and productivity of 3.2 mg L−1 h−1. This study provides further knowledge for integration of terpene bioprocesses by in situ product recovery, which could be applied for many terpene studies towards the industrialization of fragrant molecules. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Basel : MDPI AG |
|
dc.relation.ispartofseries |
Molecules 24 (2019), Nr. 18 |
|
dc.rights |
CC BY 4.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
(+)-zizaene |
eng |
dc.subject |
Chrysopogon zizanioides |
eng |
dc.subject |
Expanded bed adsorption |
eng |
dc.subject |
In situ product recovery |
eng |
dc.subject |
Khusimene |
eng |
dc.subject |
Khusimol |
eng |
dc.subject |
Sesquiterpenes |
eng |
dc.subject |
Terpenes |
eng |
dc.subject |
Vetiver essential oil |
eng |
dc.subject.ddc |
540 | Chemie
|
ger |
dc.title |
Improved production and in situ recovery of sesquiterpene (+)-zizaene from metabolically-engineered E. Coli |
|
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.issn |
14203049 |
|
dc.relation.doi |
https://doi.org/10.3390/molecules24183356 |
|
dc.bibliographicCitation.volume |
24 |
|
dc.bibliographicCitation.firstPage |
3356 |
|
dc.description.version |
publishedVersion |
|
tib.accessRights |
frei zug�nglich |
|