dc.identifier.uri |
http://dx.doi.org/10.15488/56 |
|
dc.identifier.uri |
http://www.repo.uni-hannover.de/handle/123456789/74 |
|
dc.contributor.author |
Lesker, Till
|
|
dc.contributor.author |
Maiss, Edgar
|
|
dc.date.accessioned |
2015-10-13T14:36:04Z |
|
dc.date.available |
2015-10-13T14:36:04Z |
|
dc.date.issued |
2013-10-09 |
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dc.identifier.citation |
Lesker, Till; Maiss, Edgar: In planta Protein Interactions of Three Alphacryptoviruses and Three Betacryptoviruses from White Clover, Red Clover and Dill by Bimolecular Fluorescence Complementation Analysis. In: Viruses 5 (2013), Nr. 10, S. 2512-2530. DOI: http://dx.doi.org/10.3390/v5102512 |
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dc.description.abstract |
Plant-infecting viruses of the genera Alpha- and Betacryptovirus within the family Partitiviridae cause no visible effects on their hosts and are only transmitted by cell division and through gametes. The bipartite dsRNA genome is encoding a RNA-dependent RNA polymerase (RdRp) and a coat protein (CP). Aside from sequence and structural analysis, the investigation of protein interactions is another step towards virus characterization. Therefore, ORFs of two type members White Clover Cryptic Virus 1 and 2 (WCCV-1 and WCCV-2), as well as the related viruses from Red Clover and Dill were introduced into a bimolecular fluorescence complementation assay. We showed CP-CP dimerization for all tested viruses with localization for alphacryptoviruses at the nuclear membrane and for betacryptoviruses close to cell walls within the cytoplasm. For CPs of WCCV-1 and WCCV-2, deletion mutants were created to determine internal interaction sites. Moreover, RdRp self-interaction was found for all viruses, whereas CP-RdRp interactions were only detectable for the alphacryptoviruses. An intra-genus test of CPs was successful in various virus combinations, whereas an inter-genus interaction of WCCV-1CP and WCCV-2CP was absent. This is the first report of in vivo protein interactions of members in the family Partitiviridae, indicating distinct features of the alpha- and betacryptoviruses. |
eng |
dc.description.sponsorship |
DFG |
|
dc.language.iso |
eng |
eng |
dc.publisher |
Basel : MDPI AG |
|
dc.relation.ispartofseries |
Viruses 5 (2013), Nr. 10 |
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dc.rights |
CC BY 3.0 Unported |
|
dc.rights.uri |
http://creativecommons.org/licenses/by/3.0/ |
|
dc.subject |
Partitiviridae |
eng |
dc.subject |
Alphacryptovirus |
eng |
dc.subject |
Betacryptovirus |
eng |
dc.subject |
protein interaction |
eng |
dc.subject |
Bimolecular fluorescence complementation |
eng |
dc.subject |
BiFC |
eng |
dc.subject |
White Clover |
eng |
dc.subject |
Red Clover |
eng |
dc.subject |
Dill |
eng |
dc.subject |
Partitiviridae |
ger |
dc.subject |
Alphacryptovirus |
ger |
dc.subject |
Betacryptovirus |
ger |
dc.subject |
Protein-Wechselwirkung |
ger |
dc.subject |
Bimolekulare Fluoreszenzkomplementation |
ger |
dc.subject |
BiFC |
ger |
dc.subject |
Trifolium repens |
ger |
dc.subject |
Trifolium pratense |
ger |
dc.subject |
Anethum graveolens |
ger |
dc.subject.classification |
Pflanzenviren |
ger |
dc.subject.classification |
Protein-Protein-Wechselwirkung |
ger |
dc.subject.classification |
Weißklee |
ger |
dc.subject.classification |
Rotklee |
ger |
dc.subject.classification |
Dill |
ger |
dc.subject.ddc |
570 | Biowissenschaften, Biologie
|
ger |
dc.subject.ddc |
580 | Pflanzen (Botanik)
|
ger |
dc.title |
In planta Protein Interactions of Three Alphacryptoviruses and Three Betacryptoviruses from White Clover, Red Clover and Dill by Bimolecular Fluorescence Complementation Analysis |
eng |
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.essn |
1999-4915 |
|
dc.relation.doi |
http://dx.doi.org/10.3390/v5102512 |
|
dc.bibliographicCitation.firstPage |
2512 |
|
dc.bibliographicCitation.lastPage |
2530 |
|
dc.description.version |
publishedVersion |
|
tib.accessRights |
frei zug�nglich |
|