dc.identifier.uri |
http://dx.doi.org/10.15488/678 |
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dc.identifier.uri |
http://www.repo.uni-hannover.de/handle/123456789/702 |
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dc.contributor.author |
Schmidt, Simone
|
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dc.contributor.author |
Stahl, Frank
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dc.contributor.author |
Mutz, Kai-Oliver
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dc.contributor.author |
Scheper, Thomas
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dc.contributor.author |
Hahn, Andreas
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dc.contributor.author |
Schuchardt, Jan Philipp
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dc.date.accessioned |
2016-11-09T10:37:57Z |
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dc.date.available |
2016-11-09T10:37:57Z |
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dc.date.issued |
2012 |
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dc.identifier.citation |
Schmidt, Simone; Stahl, Frank; Mutz, Kai-Oliver; Scheper, Thomas; Hahn, Andreas et al.: Transcriptome-based identification of antioxidative gene expression after fish oil supplementation in normo- and dyslipidemic men. In: Nutrition and Metabolism 9 (2012), 45. DOI: http://dx.doi.org/10.1186/1743-7075-9-45 |
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dc.description.abstract |
Background: The beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs), especially in dyslipidemic subjects with a high risk of cardiovascular disease, are widely described in the literature. A lot of effects of n-3 PUFAs and their oxidized metabolites are triggered by regulating the expression of genes. Currently, it is uncertain if the administration of n-3 PUFAs results in different expression changes of genes related to antioxidative mechanisms in normo- and dyslipidemic subjects, which may partly explain their cardioprotective effects. The aim of this study was to investigate the effects of n-3 PUFA supplementation on expression changes of genes involved in oxidative processes. Methods: Ten normo- and ten dyslipidemic men were supplemented for twelve weeks with fish oil capsules, providing 1.14 g docosahexaenoic acid and 1.56 g eicosapentaenoic acid. Gene expression levels were determined by whole genome microarray analysis and quantitative real-time polymerase chain reaction (qRT-PCR). Results: Using microarrays, we discovered an increased expression of antioxidative enzymes and a decreased expression of pro-oxidative and tissue enzymes, such as cytochrome P450 enzymes and matrix metalloproteinases, in both normo- and dyslipidemic men. An up-regulation of catalase and heme oxigenase 2 in both normo- and dyslipidemic subjects and an up-regulation of cytochrome P450 enzyme 1A2 only in dyslipidemic subjects could be observed by qRT-PCR analysis. Conclusions: Supplementation of normo- and dyslipidemic subjects with n-3 PUFAs changed the expression of genes related to oxidative processes, which may suggest antioxidative and potential cardioprotective effects of n-3 PUFAs. Further studies combining genetic and metabolic endpoints are needed to verify the regulative effects of n-3 PUFAs in antioxidative gene expression to better understand their beneficial effects in health and disease prevention. |
eng |
dc.description.sponsorship |
BMBF |
|
dc.language.iso |
eng |
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dc.publisher |
London : BioMed Central Ltd. |
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dc.relation.ispartofseries |
Nutrition and Metabolism 9 (2012) |
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dc.rights |
CC BY 2.0 Unported |
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dc.rights.uri |
https://creativecommons.org/licenses/by/2.0/ |
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dc.subject |
Antioxidative defence |
eng |
dc.subject |
Catalase |
eng |
dc.subject |
Cytochrome P450 enzyme |
eng |
dc.subject |
Dyslipidemia |
eng |
dc.subject |
Glutathione |
eng |
dc.subject |
Heme oxygenase |
eng |
dc.subject |
Matrix metalloproteinase |
eng |
dc.subject |
Omega-3 fatty acids |
eng |
dc.subject |
Oxylipines |
eng |
dc.subject |
catalase |
eng |
dc.subject |
cytochrome P450 |
eng |
dc.subject |
cytochrome P450 1A2 |
eng |
dc.subject |
docosahexaenoic acid |
eng |
dc.subject |
gelatinase A |
eng |
dc.subject |
glutathione reductase |
eng |
dc.subject |
glutathione transferase |
eng |
dc.subject |
heme oxygenase 2 |
eng |
dc.subject |
icosapentaenoic acid |
eng |
dc.subject |
immunoglobulin enhancer binding protein |
eng |
dc.subject |
matrix metalloproteinase |
eng |
dc.subject |
matrix metalloproteinase 25 |
eng |
dc.subject |
mitogen activated protein kinase |
eng |
dc.subject |
omega 3 fatty acid |
eng |
dc.subject |
stromelysin |
eng |
dc.subject |
superoxide dismutase |
eng |
dc.subject |
transcriptome |
eng |
dc.subject |
unclassified drug |
eng |
dc.subject |
adult |
eng |
dc.subject |
antioxidant activity |
eng |
dc.subject |
article |
eng |
dc.subject |
clinical article |
eng |
dc.subject |
controlled study |
eng |
dc.subject |
down regulation |
eng |
dc.subject |
drug effect |
eng |
dc.subject |
dyslipidemia |
eng |
dc.subject |
erythrocyte membrane |
eng |
dc.subject |
fatty acid analysis |
eng |
dc.subject |
gene control |
eng |
dc.subject |
gene expression |
eng |
dc.subject |
genetic transcription |
eng |
dc.subject |
heart protection |
eng |
dc.subject |
human |
eng |
dc.subject |
male |
eng |
dc.subject |
microarray analysis |
eng |
dc.subject |
nucleotide sequence |
eng |
dc.subject |
oxidative stress |
eng |
dc.subject |
randomized controlled trial |
eng |
dc.subject |
real time polymerase chain reaction |
eng |
dc.subject |
upregulation |
eng |
dc.subject.ddc |
570 | Biowissenschaften, Biologie
|
ger |
dc.subject.ddc |
610 | Medizin, Gesundheit
|
ger |
dc.title |
Transcriptome-based identification of antioxidative gene expression after fish oil supplementation in normo- and dyslipidemic men |
eng |
dc.type |
Article |
|
dc.type |
Text |
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dc.relation.issn |
1743-7075 |
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dc.relation.doi |
http://dx.doi.org/10.1186/1743-7075-9-45 |
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dc.bibliographicCitation.volume |
9 |
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dc.bibliographicCitation.firstPage |
45 |
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dc.description.version |
publishedVersion |
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tib.accessRights |
frei zug�nglich |
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