dc.identifier.uri |
http://dx.doi.org/10.15488/3188 |
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dc.identifier.uri |
http://www.repo.uni-hannover.de/handle/123456789/3218 |
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dc.contributor.author |
Greupner, Theresa
|
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dc.contributor.author |
Kutzner, Laura
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dc.contributor.author |
Nolte, Fabian
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dc.contributor.author |
Strangmann, Alena
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dc.contributor.author |
Kohrs, Heike
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dc.contributor.author |
Hahn, Andreas
|
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dc.contributor.author |
Schebb, Nils Helge
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dc.contributor.author |
Schuchardt, Jan Philipp
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dc.date.accessioned |
2018-04-27T12:01:40Z |
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dc.date.available |
2018-04-27T12:01:40Z |
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dc.date.issued |
2018 |
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dc.identifier.citation |
Greupner, T.; Kutzner, L.; Nolte, F.; Strangmann, A.; Kohrs, H. et al.: Effects of a 12-week high-α-linolenic acid intervention on EPA and DHA concentrations in red blood cells and plasma oxylipin pattern in subjects with a low EPA and DHA status. In: Food and Function 9 (2018), Nr. 3, S. 1587-1600. DOI: https://doi.org/10.1039/c7fo01809f |
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dc.description.abstract |
The essential omega-3 fatty acid alpha-linolenic acid (ALA, 18:3n3) can be converted into EPA and DHA. The aim of the present study was to determine the effect of a high-ALA diet on EPA and DHA levels in red blood cells (RBCs) and their oxylipins in the plasma of subjects with a low EPA and DHA status. Fatty acid concentrations [μg mL−1] and relative amounts [% of total fatty acids] in the RBCs of 19 healthy men (mean age 26.4 ± 4.6 years) were analyzed by means of GC-FID. Free plasma oxylipin concentrations were determined by LC-MS based targeted metabolomics. Samples were collected and analyzed at baseline (week 0) and after 1 (week 1), 3 (week 3), 6 (week 6), and 12 (week 12) weeks of high dietary ALA intake (14.0 ± 0.45 g day−1). ALA concentrations significantly (p < 0.001) increased from 1.44 ± 0.10 (week 0) to 4.65 ± 0.22 (week 1), 5.47 ± 0.23 (week 3), 6.25 ± 0.24 (week 6), and 5.80 ± 0.28 (week 12) μg mL−1. EPA concentrations increased from 6.13 ± 0.51 (week 0) to 7.33 ± 0.33 (week 1), 8.38 ± 0.42 (p = 0.021, week 3), 10.9 ± 0.67 (p < 0.001, week 6), and 11.0 ± 0.64 (p < 0.001, week 12) μg mL−1. DHA concentrations unexpectedly decreased from 41.0 ± 1.93 (week 0) to 37.0 ± 1.32 (week 1), 36.1 ± 1.37 (week 3), 35.1 ± 1.06 (p = 0.010, week 6), and 30.4 ± 1.09 (p < 0.001, week 12) μg mL−1. Relative ΣEPA + DHA amounts were unchanged during the intervention (week 0: 4.63 ± 0.19, week 1: 4.67 ± 0.16, week 3: 4.61 ± 0.13, week 6: 4.73 ± 0.15, week 12: 4.52 ± 0.11). ALA- and EPA-derived hydroxy- and dihydroxy-PUFA increased similarly to their PUFA precursors, although in the case of ALA-derived oxylipins, the concentrations increased less rapidly and to a lesser extent compared to the concentrations of their precursor FA. LA-derived oxylipins remained unchanged and arachidonic acid and DHA oxylipin concentrations were not significantly changed. Our results confirm that the intake of ALA is not a sufficient source for the increase of EPA + DHA in subjects on a Western diet. Specifically, a high-ALA diet results in increased EPA and declined DHA concentrations. However, the changes effectively balance each other out so that ΣEPA + DHA in RBCs – which is an established marker for health protective effects of omega-3-PUFA – remains constant. The PUFA levels in RBCs reflect the concentration and its changes in plasma hydroxy- and dihydroxy-PUFA concentrations for ALA and EPA. |
eng |
dc.language.iso |
eng |
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dc.publisher |
Cambridge : Royal Society of Chemistry |
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dc.relation.ispartofseries |
Food and Function 9 (2018), Nr. 3 |
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dc.rights |
CC BY 3.0 Unported |
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dc.rights.uri |
https://creativecommons.org/licenses/by/3.0/ |
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dc.subject |
Blood |
eng |
dc.subject |
Cells |
eng |
dc.subject |
Chemical analysis |
eng |
dc.subject |
Acid concentrations |
eng |
dc.subject |
Alpha linolenic acids |
eng |
dc.subject |
Arachidonic acids |
eng |
dc.subject |
Mean ages |
eng |
dc.subject |
Metabolomics |
eng |
dc.subject |
Omega-3-fatty acids |
eng |
dc.subject |
Protective effects |
eng |
dc.subject |
Red blood cell |
eng |
dc.subject |
Fatty acids |
eng |
dc.subject.ddc |
660 | Technische Chemie
|
ger |
dc.title |
Effects of a 12-week high-α-linolenic acid intervention on EPA and DHA concentrations in red blood cells and plasma oxylipin pattern in subjects with a low EPA and DHA status |
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dc.type |
Article |
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dc.type |
Text |
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dc.relation.issn |
2042-6496 |
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dc.relation.doi |
https://doi.org/10.1039/c7fo01809f |
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dc.bibliographicCitation.issue |
3 |
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dc.bibliographicCitation.volume |
9 |
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dc.bibliographicCitation.firstPage |
1587 |
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dc.bibliographicCitation.lastPage |
1600 |
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dc.description.version |
publishedVersion |
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tib.accessRights |
frei zug�nglich |
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