dc.identifier.uri |
http://dx.doi.org/10.15488/3142 |
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dc.identifier.uri |
http://www.repo.uni-hannover.de/handle/123456789/3172 |
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dc.contributor.author |
Andric, Nadine
|
|
dc.contributor.author |
Ehmke, Tobias
|
|
dc.contributor.author |
Stumpp, Nico S.
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|
dc.contributor.author |
Ripken, Tammo
|
|
dc.contributor.author |
Heisterkamp, Alexander
|
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dc.contributor.author |
Stiesch, Meike
|
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dc.date.accessioned |
2018-04-19T06:54:24Z |
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dc.date.available |
2018-04-19T06:54:24Z |
|
dc.date.issued |
2016 |
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dc.identifier.citation |
Andric, N.; Ehmke, T.; Stumpp, N.S.; Ripken, T.; Heisterkamp, A. et al.: Nonlinear laser scanning microscopy of oral multispecies-biofilms: Fixative induced fluorescence as a fast and economical in vitro screening method. In: BioNanoMaterials 17 (2016), Nr. Februar, S. 73-77. DOI: https://doi.org/10.1515/bnm-2015-0028 |
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dc.description.abstract |
In this letter we report a fast and easy method which could be used for initial screening of multispecies-biofilm development on putative new dental implant materials. Most staining methods require numerous washing steps that can result in detachment of loosely bound biofilms and therefore falsify the results. Thus, we used glutaraldehyde fixation, which induces autofluorescence through bacterial membrane protein cross-linking and concurrently stabilizes the biofilm structure. We analyzed the biofilms with nonlinear laser scanning microscopy and were able to (I) evaluate the multispecies-biofilm growth and (II) distinguish between bacterial species based on different two-photon autofluorescence intensities. © 2016 by De Gruyter. |
eng |
dc.language.iso |
eng |
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dc.publisher |
Berlin : De Gruyter |
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dc.relation.ispartofseries |
BioNanoMaterials 17 (2016), Nr. Februar |
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dc.rights |
Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich. |
|
dc.subject |
autofluorescence |
eng |
dc.subject |
dental implant |
eng |
dc.subject |
glutaraldehyde |
eng |
dc.subject |
multiphoton microscopy |
eng |
dc.subject |
periimplantitis |
eng |
dc.subject |
Schiff base |
eng |
dc.subject |
bacterial protein |
eng |
dc.subject |
cell membrane protein |
eng |
dc.subject |
fixative |
eng |
dc.subject |
glutaraldehyde |
eng |
dc.subject |
sodium chloride |
eng |
dc.subject |
Actinomyces naeslundii |
eng |
dc.subject |
Article |
eng |
dc.subject |
autofluorescence |
eng |
dc.subject |
bacterial membrane |
eng |
dc.subject |
biofilm |
eng |
dc.subject |
controlled study |
eng |
dc.subject |
fluorescence in situ hybridization |
eng |
dc.subject |
Gram negative bacterium |
eng |
dc.subject |
in vitro study |
eng |
dc.subject |
laser microscopy |
eng |
dc.subject |
nonhuman |
eng |
dc.subject |
nonlinear laser scanning microscopy |
eng |
dc.subject |
priority journal |
eng |
dc.subject |
protein cross linking |
eng |
dc.subject |
screening |
eng |
dc.subject |
staining |
eng |
dc.subject |
Streptococcus oralis |
eng |
dc.subject |
tooth implant |
eng |
dc.subject |
Veillonella dispar |
eng |
dc.subject.ddc |
610 | Medizin, Gesundheit
|
ger |
dc.title |
Nonlinear laser scanning microscopy of oral multispecies-biofilms: Fixative induced fluorescence as a fast and economical in vitro screening method |
|
dc.type |
Article |
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dc.type |
Text |
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dc.relation.issn |
2193-0651 |
|
dc.relation.doi |
https://doi.org/10.1515/bnm-2015-0028 |
|
dc.bibliographicCitation.issue |
Februar |
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dc.bibliographicCitation.volume |
17 |
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dc.bibliographicCitation.firstPage |
73 |
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dc.bibliographicCitation.lastPage |
77 |
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dc.description.version |
publishedVersion |
|
tib.accessRights |
frei zug�nglich |
|