Zusammenfassung: | |
Xanthophylls have attracted a lot of interest since their health benefits were documented. Unfortunately, studying their intestinal absorption is often affected by high baseline levels present in the fasting plasma. As a-cryptoxanthin is rarely found in the traditional European diet, its concentration in human plasma is extremely low. A pilot human intervention study was designed using a-cryptoxanthin for the first time as a marker xanthophyll in a minimally formulated cellulose-based supplement. α-Cryptoxanthin was administered in gelatin soft-gel capsules in multiple doses of 156 μg/d to three male volunteers (age 27.3 (SD 4.7) years; BMI 21.6 (SD 0.3) kg/m2) for 16 d after a 2-week carotenoid depletion period. Fasting blood samples were taken before the intervention and after 3, 6, 9, 13 and 16 d. Plasma HPLC analyses allowed for determination of the concentration; liquid chromatography-MS in the single ion monitoring mode was used to confirm peak assignment. The concentrations of α-cryptoxanthin increased significantly after only 3 d of supplementation. The concentration-time plots showed a characteristic shape with a first maximum after day 6, a decline until day 9 and a gradual second rise until the end of the study. standardisation of plasma α-cryptoxanthin concentrations to triacylglycerol or total cholesterol did not influence the characteristics. The maximum concentrations reached at the end of the intervention period ranged from 0.077 to 0.160 μmol/l. These results suggest a high intestinal absorption and an enrichment of α-cryptoxanthin in the plasma even from a minimally formulated cellulose-based supplement. © The Authors 2006.
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Lizenzbestimmungen: | Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich. |
Publikationstyp: | Article |
Publikationsstatus: | publishedVersion |
Erstveröffentlichung: | 2006 |
Schlagwörter (englisch): | α-Cryptoxanthin, Liquid chromatography-mass spectroscopy in the single ion monitoring mode, Pilot studies, Plasma xanthophyll response, alpha cryptoxanthin, carotenoid, cellulose, cholesterol, cryptoxanthin, gelatin, triacylglycerol, unclassified drug, xanthophyll, beta carotene, cryptoxanthin, drug derivative, adult, article, blood sampling, cholesterol blood level, drug absorption, drug blood level, drug capsule, drug formulation, drug isolation, high performance liquid chromatography, human, intestine absorption, liquid chromatography, male, mass spectrometry, multiple drug dose, pilot study, anthropometry, blood, drug administration, isolation and purification, methodology, microcapsule, oral drug administration, Administration, Oral, Adult, Anthropometry, beta Carotene, Capsules, Chromatography, High Pressure Liquid, Chromatography, Liquid, Drug Administration Schedule, Humans, Intestinal Absorption, Male, Mass Spectrometry, Pilot Projects, Xanthophylls |
Fachliche Zuordnung (DDC): | 610 | Medizin, Gesundheit |
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