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Originalpublikation
Wetzel, C.; Jansen-Olliges, L.; Stadler, M.; Surup, F.; Zeilinger, C. et al.: Analysis of SARS-CoV-2 spike RBD binding to ACE2 and its inhibition by fungal cohaerin C using surface enhanced Raman spectroscopy. In: Biomedical Optics Express 14 (2023), Nr. 8, 4097. DOI: https://doi.org/10.1364/boe.495685
Version im Repositorium
Zum Zitieren der Version im Repositorium verwenden Sie bitte diesen DOI:
https://doi.org/10.15488/17297
| Zusammenfassung: | |
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The structure of the SARS-CoV-2 spike RBD and human ACE2 as well as changes in the structure due to binding activities were analysed using surface enhanced Raman spectroscopy. The inhibitor cohaerin C was applied to inhibit the binding between spike RBD and ACE2. Differences and changes in the Raman spectra were determined using deconvolution of the amide bands and principal component analysis. We thus demonstrate a fast and label-free analysis of the protein structures and the differentiation between bound and unbound states. The approach is suitable for sensing and screening and might be relevant to investigate other protein systems as well.
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| Lizenzbestimmungen: | CC BY 4.0 Unported - https://creativecommons.org/licenses/by/4.0 |
| Publikationstyp: | Article |
| Publikationsstatus: | publishedVersion |
| Erstveröffentlichung: | 2023 |
| Schlagwörter (englisch): | Amides, Diseases, Light transmission, Principal component analysis, Proteins, Raman spectroscopy, Binding activities, Bound-states, Deconvolutions, Label free analysis, Principal-component analysis, Protein system, Proteins structures, Surface enhanced Raman spectroscopy, Unbound state, Coronavirus |
| Fachliche Zuordnung (DDC): | 530 | Physik, 610 | Medizin, Gesundheit |
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