dc.identifier.uri |
http://dx.doi.org/10.15488/15334 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/15454 |
|
dc.contributor.author |
Meyer, Katharina V.
|
|
dc.contributor.author |
Winkler, Steffen
|
|
dc.contributor.author |
Lienig, Pascal
|
|
dc.contributor.author |
Dräger, Gerald
|
|
dc.contributor.author |
Bahnemann, Janina
|
|
dc.date.accessioned |
2023-11-16T08:09:24Z |
|
dc.date.available |
2023-11-16T08:09:24Z |
|
dc.date.issued |
2023 |
|
dc.identifier.citation |
Meyer, K.V.; Winkler, S.; Lienig, P.; Dräger, G.; Bahnemann, J.: 3D-Printed Microfluidic Perfusion System for Parallel Monitoring of Hydrogel-Embedded Cell Cultures. In: Cells 12 (2023), Nr. 14, 1816. DOI: https://doi.org/10.3390/cells12141816 |
|
dc.description.abstract |
The use of three-dimensional (3D) cell cultures has become increasingly popular in the contexts of drug discovery, disease modelling, and tissue engineering, as they aim to replicate in vivo-like conditions. To achieve this, new hydrogels are being developed to mimic the extracellular matrix. Testing the ability of these hydrogels is crucial, and the presented 3D-printed microfluidic perfusion system offers a novel solution for the parallel cultivation and evaluation of four separate 3D cell cultures. This system enables easy microscopic monitoring of the hydrogel-embedded cells and significantly reduces the required volumes of hydrogel and cell suspension. This cultivation device is comprised of two 3D-printed parts, which provide four cell-containing hydrogel chambers and the associated perfusion medium chambers. An interfacing porous membrane ensures a defined hydrogel thickness and prevents flow-induced hydrogel detachment. Integrated microfluidic channels connect the perfusion chambers to the overall perfusion system, which can be operated in a standard CO2-incubator. A 3D-printed adapter ensures the compatibility of the cultivation device with standard imaging systems. Cultivation and cell staining experiments with hydrogel-embedded murine fibroblasts confirmed that cell morphology, viability, and growth inside this cultivation device are comparable with those observed within standard 96-well plates. Due to the high degree of customization offered by additive manufacturing, this system has great potential to be used as a customizable platform for 3D cell culture applications. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Basel : MDPI |
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dc.relation.ispartofseries |
Cells 12 (2023), Nr. 14 |
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dc.rights |
CC BY 4.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0 |
|
dc.subject |
3D cell culture |
eng |
dc.subject |
3D printing |
eng |
dc.subject |
hydrogel |
eng |
dc.subject |
membrane integration |
eng |
dc.subject |
microfluidic perfusion system |
eng |
dc.subject |
organ-on-chip |
eng |
dc.subject.ddc |
570 | Biowissenschaften, Biologie
|
|
dc.title |
3D-Printed Microfluidic Perfusion System for Parallel Monitoring of Hydrogel-Embedded Cell Cultures |
eng |
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.essn |
2073-4409 |
|
dc.relation.doi |
https://doi.org/10.3390/cells12141816 |
|
dc.bibliographicCitation.issue |
14 |
|
dc.bibliographicCitation.volume |
12 |
|
dc.bibliographicCitation.firstPage |
1816 |
|
dc.description.version |
publishedVersion |
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tib.accessRights |
frei zug�nglich |
|