dc.identifier.uri |
http://dx.doi.org/10.15488/13904 |
|
dc.identifier.uri |
https://www.repo.uni-hannover.de/handle/123456789/14018 |
|
dc.contributor.author |
Heuer, Christopher
|
|
dc.contributor.author |
Preuss, John-Alexander
|
|
dc.contributor.author |
Buttkewitz, Marc
|
|
dc.contributor.author |
Scheper, Thomas
|
|
dc.contributor.author |
Segal, Ester
|
|
dc.contributor.author |
Bahnemann, Janina
|
|
dc.date.accessioned |
2023-06-23T06:48:30Z |
|
dc.date.available |
2023-06-23T06:48:30Z |
|
dc.date.issued |
2022 |
|
dc.identifier.citation |
Heuer, C.; Preuss, J.-A.; Buttkewitz, M.; Scheper, T.; Segal, E. et al.: A 3D-printed microfluidic gradient generator with integrated photonic silicon sensors for rapid antimicrobial susceptibility testing. In: Lab on a Chip 22 (2022), Nr. 24, S. 4950-4961. DOI: https://doi.org/10.1039/d2lc00640e |
|
dc.description.abstract |
With antimicrobial resistance becoming a major threat to healthcare settings around the world, there is a paramount need for rapid point-of-care antimicrobial susceptibility testing (AST) diagnostics. Unfortunately, most currently available clinical AST tools are lengthy, laborious, or are simply inappropriate for point-of-care testing. Herein, we design a 3D-printed microfluidic gradient generator that automatically produces two-fold dilution series of clinically relevant antimicrobials. We first establish the compatibility of these generators for classical AST (i.e., broth microdilution) and then extend their application to include a complete on-chip label-free and phenotypic AST. This is accomplished by the integration of photonic silicon chips, which provide a preferential surface for microbial colonization and allow optical tracking of bacterial behavior and growth at a solid-liquid interface in real-time by phase shift reflectometric interference spectroscopic measurements (PRISM). Using Escherichia coli and ciprofloxacin as a model pathogen-drug combination, we successfully determine the minimum inhibitory concentration within less than 90 minutes. This gradient generator-based PRISM assay provides an integrated AST device that is viable for convenient point-of-care testing and offers a promising and most importantly, rapid alternative to current clinical practices, which extend to 8-24 h. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Cambridge : RSC |
|
dc.relation.ispartofseries |
Lab on a Chip 22 (2022), Nr. 24 |
|
dc.rights |
CC BY-NC 3.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc/3.0 |
|
dc.subject |
Microfluidics |
eng |
dc.subject |
Silicon |
eng |
dc.subject |
3D printers |
eng |
dc.subject |
Escherichia coli |
eng |
dc.subject |
Phase interfaces |
eng |
dc.subject.ddc |
004 | Informatik
|
ger |
dc.subject.ddc |
570 | Biowissenschaften, Biologie
|
ger |
dc.subject.ddc |
540 | Chemie
|
ger |
dc.title |
A 3D-printed microfluidic gradient generator with integrated photonic silicon sensors for rapid antimicrobial susceptibility testing |
eng |
dc.type |
Article |
|
dc.type |
Text |
|
dc.relation.essn |
1473-0189 |
|
dc.relation.issn |
1473-0197 |
|
dc.relation.doi |
https://doi.org/10.1039/d2lc00640e |
|
dc.bibliographicCitation.issue |
24 |
|
dc.bibliographicCitation.volume |
22 |
|
dc.bibliographicCitation.firstPage |
4950 |
|
dc.bibliographicCitation.lastPage |
4961 |
|
dc.description.version |
publishedVersion |
|
tib.accessRights |
frei zug�nglich |
|