dc.identifier.uri |
http://dx.doi.org/10.15488/1324 |
|
dc.identifier.uri |
http://www.repo.uni-hannover.de/handle/123456789/1349 |
|
dc.contributor.author |
Glyk, Anna
|
|
dc.contributor.author |
Heinisch, Sandra L.
|
|
dc.contributor.author |
Scheper, Thomas
|
|
dc.contributor.author |
Beutel, Sascha
|
|
dc.date.accessioned |
2017-04-20T08:42:18Z |
|
dc.date.available |
2017-04-20T08:42:18Z |
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dc.date.issued |
2015 |
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dc.identifier.citation |
Glyk, A.; Heinisch, S.L.; Scheper, T.; Beutel, S.: Comparison of colorimetric methods for the quantification of model proteins in aqueous two-phase systems. In: Analytical Biochemistry 477 (2015), S. 35-37. DOI: https://doi.org/10.1016/j.ab.2015.02.007 |
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dc.description.abstract |
In the current study, the quantification of different model proteins in the presence of typical aqueous two-phase system components was investigated by using the Bradford and bicinchoninic acid (BCA) assays. Each phase-forming component above 1 and 5 wt% had considerable effects on the protein quantification in both assays, respectively, resulting in diminished protein recoveries/absorption values by increasing poly(ethylene glycol) (PEG)/salt concentration and PEG molecular weight. Therefore, a convenient dilution of both components (up to 1 and 5 wt%) before protein quantification is recommended in both assays, respectively, where the BCA assay is favored in comparison with the Bradford assay. |
eng |
dc.language.iso |
eng |
|
dc.publisher |
Cambridge, MA : Elsevier Academic Press |
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dc.relation.ispartofseries |
Analytical Biochemistry 477 (2015) |
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dc.rights |
CC BY-NC-ND 4.0 Unported |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
dc.subject |
Aqueous two-phase system |
eng |
dc.subject |
BCA assay |
eng |
dc.subject |
Bradford assay |
eng |
dc.subject |
Colorimetric analysis |
eng |
dc.subject |
Protein quantification |
eng |
dc.subject |
citrate sodium |
eng |
dc.subject |
citric acid |
eng |
dc.subject |
dextran |
eng |
dc.subject |
drug analysis |
eng |
dc.subject |
macrogol |
eng |
dc.subject |
macrogol derivative |
eng |
dc.subject |
phosphate |
eng |
dc.subject |
potassium derivative |
eng |
dc.subject |
potassium dihydrogen phosphate |
eng |
dc.subject |
water |
eng |
dc.subject |
alkalinity |
eng |
dc.subject |
bicinchoninic acid assay |
eng |
dc.subject |
chemical phenomena |
eng |
dc.subject |
chemistry |
eng |
dc.subject |
colorimetry |
eng |
dc.subject |
comparative study |
eng |
dc.subject |
intermethod comparison |
eng |
dc.subject |
limit of quantitation |
eng |
dc.subject |
molecular weight |
eng |
dc.subject |
priority journal |
eng |
dc.subject |
procedures |
eng |
dc.subject |
protein binding |
eng |
dc.subject |
protein determination |
eng |
dc.subject |
viscosity |
eng |
dc.subject |
analysis |
eng |
dc.subject |
chemistry |
eng |
dc.subject |
Citrates |
eng |
dc.subject |
Phosphates |
eng |
dc.subject |
Polyethylene Glycols |
eng |
dc.subject |
Potassium Compounds |
eng |
dc.subject |
Proteins |
eng |
dc.subject.ddc |
500 | Naturwissenschaften
|
ger |
dc.subject.ddc |
570 | Biowissenschaften, Biologie
|
ger |
dc.subject.ddc |
540 | Chemie
|
ger |
dc.title |
Comparison of colorimetric methods for the quantification of model proteins in aqueous two-phase systems |
eng |
dc.type |
Article |
|
dc.type |
Text |
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dc.relation.essn |
0003-2697 |
|
dc.relation.doi |
10.1016/j.ab.2015.02.007 |
|
dc.bibliographicCitation.volume |
477 |
|
dc.bibliographicCitation.firstPage |
35 |
|
dc.bibliographicCitation.lastPage |
37 |
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dc.description.version |
acceptedVersion |
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tib.accessRights |
frei zug�nglich |
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