Analysis and Design of Stimulus Response Curves of E. coli

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Kremling, Andreas; Goehler, Anna; Jahreis, Knut; Nees, Markus; Auerbach, Benedikt et al.: Analysis and Design of Stimulus Response Curves of E. coli. In: Metabolites 2 (2012), Nr. 4, S. 844-871. DOI: https://doi.org/10.3390/metabo2040844

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Abstract: 
Metabolism and signalling are tightly coupled in bacteria. Combining several theoretical approaches, a core model is presented that describes transcriptional and allosteric control of glycolysis in Escherichia coli. Experimental data based on microarrays, signalling components and extracellular metabolites are used to estimate kinetic parameters. A newly designed strain was used that adjusts the incoming glucose flux into the system and allows a kinetic analysis. Based on the results, prediction for intracelluar metabolite concentrations over a broad range of the growth rate could be performed and compared with data from literature.
License of this version: CC BY 3.0
Document Type: article
Publishing status: publishedVersion
Issue Date: 2012
Appears in Collections:Naturwissenschaftliche Fakultät

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1 image of flag of Germany Germany 5 100.00%

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