Ude, C.; Hentrop, T.; Lindner, P.; Lücking, T.H.; Scheper, T.; Beutel, S.: New perspectives in shake flask pH control using a 3D-printed control unit based on pH online measurement. In: Sensors and Actuators, B: Chemical 221 (2015), S. 1035-1043. DOI: https://doi.org/10.1016/j.snb.2015.07.017
Abstract: | |
Abstract Online pH control during microbial shake flask cultivation has not been established due to the lack of a practical combination of an online sensor system and an appropriate control unit. The objective of this investigation was to develop a minimum scale dosage apparatus, namely shake flask controller ("SFC"), which can control the pH during a complete cultivation and serves as technical example for the application of small liquid dispensing lab devices. A well evaluated optical, chemosensor based, noninvasive, multisensory platform prototype for online DO (dissolved oxygen)-, pH- and biomass measurement served as sensor. The SFC was designed as cap-integrated, semi-autarkical control unit. Minimum scale working parts like the commercial mp6 piezoelectric micropumps and miniature solenoid valves were combined with a selective laser sintering (SLS) printed backbone. In general it is intended to extend its application range on the control of enzymatic assays, polymerization processes, cell disruption methods or the precise dispense of special chemicals like inducers or inhibitors. It could be proved that pH control within a range of 0.1 pH units could be maintained at different cultivation conditions. A proportional-integral-derivative- (PID) controller and an adaptive proportional controller were successfully applied to calculate the balancing solution volume. SLS based 3D printing using polyamide combined with state-of-the-art micro pumps proved to be perfectly adaptable for minimum size, autoclavable lab devices. | |
License of this version: | CC BY-NC-ND 4.0 Unported |
Document Type: | Article |
Publishing status: | acceptedVersion |
Issue Date: | 2015 |
Appears in Collections: | Naturwissenschaftliche Fakultät |
pos. | country | downloads | ||
---|---|---|---|---|
total | perc. | |||
1 | Germany | 191 | 48.60% | |
2 | United States | 65 | 16.54% | |
3 | Russian Federation | 17 | 4.33% | |
4 | China | 16 | 4.07% | |
5 | France | 15 | 3.82% | |
6 | India | 12 | 3.05% | |
7 | United Kingdom | 8 | 2.04% | |
8 | Japan | 6 | 1.53% | |
9 | Canada | 6 | 1.53% | |
10 | No geo information available | 5 | 1.27% | |
other countries | 52 | 13.23% |
Hinweis
Zur Erhebung der Downloadstatistiken kommen entsprechend dem „COUNTER Code of Practice for e-Resources“ international anerkannte Regeln und Normen zur Anwendung. COUNTER ist eine internationale Non-Profit-Organisation, in der Bibliotheksverbände, Datenbankanbieter und Verlage gemeinsam an Standards zur Erhebung, Speicherung und Verarbeitung von Nutzungsdaten elektronischer Ressourcen arbeiten, welche so Objektivität und Vergleichbarkeit gewährleisten sollen. Es werden hierbei ausschließlich Zugriffe auf die entsprechenden Volltexte ausgewertet, keine Aufrufe der Website an sich.