Koch, M.; Suhr, C.; Roth, B.; Meinhardt-Wollweber, M.: Iterative morphological and mollifier-based baseline correction for Raman spectra. In: Journal of Raman Spectroscopy 48 (2016), Nr. 2, S. 336-342. DOI: https://doi.org/10.1002/jrs.5010
Zusammenfassung: | |
In vivo Raman spectroscopy with low signal-to-noise ratio and strong, irregularly shaped fluorescence background imposes a challenge for automatic baseline correction methods. In this work, an approach that enables fast and efficient batch baseline correction has been developed, which is based on a morphological operation in combination with a mollifier algorithm. As this algorithm relies only on three parameters, which are determined by the given experimental conditions, it can be used for automatic and objective processing of many Raman spectra. The applicability of the baseline correction is demonstrated on resonance Raman spectra of beta-carotene mixed with fluorescent red ink as model system, on carotenoids in human skin, and on an excitation–emission map of the green alga Haematococcus pluvialis. In the future, the algorithm opens the potential for wide application in Raman spectra analysis in biological contexts. In particular, it greatly facilitates data processing in cases where special photochemical sample preparation or complex experimental baseline removal was required before. Similarly, processing data of experiments using resonant excitation techniques yielding strong fluorescence background is possible. This is the peer reviewed version of the following article: Koch, M.; Suhr, C.; Roth, B.; Meinhardt-Wollweber, M.: Iterative morphological and mollifier-based baseline correction for Raman spectra. In: Journal of Raman Spectroscopy 48 (2016), Nr. 2, S. 336-342, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jrs.5010/abstract. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | |
Lizenzbestimmungen: | Es gilt deutsches Urheberrecht. Das Dokument darf zum eigenen Gebrauch kostenfrei genutzt, aber nicht im Internet bereitgestellt oder an Außenstehende weitergegeben werden. |
Publikationstyp: | Article |
Publikationsstatus: | acceptedVersion |
Erstveröffentlichung: | 2016 |
Die Publikation erscheint in Sammlung(en): | Fakultät für Maschinenbau Fakultät für Mathematik und Physik |
Pos. | Land | Downloads | ||
---|---|---|---|---|
Anzahl | Proz. | |||
1 | Germany | 186 | 54,55% | |
2 | United States | 42 | 12,32% | |
3 | China | 41 | 12,02% | |
4 | Russian Federation | 6 | 1,76% | |
5 | France | 6 | 1,76% | |
6 | No geo information available | 5 | 1,47% | |
7 | Korea, Republic of | 5 | 1,47% | |
8 | Canada | 5 | 1,47% | |
9 | Czech Republic | 3 | 0,88% | |
10 | Brazil | 3 | 0,88% | |
andere | 39 | 11,44% |
Hinweis
Zur Erhebung der Downloadstatistiken kommen entsprechend dem „COUNTER Code of Practice for e-Resources“ international anerkannte Regeln und Normen zur Anwendung. COUNTER ist eine internationale Non-Profit-Organisation, in der Bibliotheksverbände, Datenbankanbieter und Verlage gemeinsam an Standards zur Erhebung, Speicherung und Verarbeitung von Nutzungsdaten elektronischer Ressourcen arbeiten, welche so Objektivität und Vergleichbarkeit gewährleisten sollen. Es werden hierbei ausschließlich Zugriffe auf die entsprechenden Volltexte ausgewertet, keine Aufrufe der Website an sich.