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Downstream processing of high chain length polysialic acid using membrane adsorbers and clay minerals for application in tissue engineering

Bice, Ismet; Celik, Hilal; Wolff, Christopher; Beutel, Sascha; Zahid, Maria; Hitzmann, Bernd; Rinas, Ursula; Kasper, Cornelia; Gerardy-Schahn, Rita; Scheper, Thomas (Weinheim : Wiley-VCH Verlag, 2013)

Polysialic acid (polySia) is a carbohydrate polymer of varying chain length. It is a promising scaffold material for tissue engineering. In this work, high chain length polySia was produced by an Escherichia coli K1 strain ...
Single-use membrane adsorbers for endotoxin removal and purification of endogenous polysialic acid from Escherichia coli K1

de Vries, Ingo; Schreiber, Sarah; Boßmann, Daniel; Hellmann, Zawadi; Kopatz, Jens; Neumann, Harald; Beutel, Sascha (Amsterdam : Elsevier B.V., 2018)

Polysialic acid (polySia) is a promising molecule for various medical applications (e.g., treatment of inflammatory neurodegenerative diseases). In this study a complete production process for human-identical α-(2,8)-linked ...
Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli

Rueda, Fabian; Céspedes, Maria Virtudes; Sánchez-Chardi, Alejandro; Seras-Franzoso, Joaquin; Pesarrodona, Mireia; Ferrer-Miralles, Neus; Vázquez, Esther; Rinas, Ursula; Unzueta, Ugutz; Mamat, Uwe; Mangues, Ramon; García-Fruitós, Elena; Villaverde, Antonio (London : BioMed Central Ltd., 2016)

Background: Production of recombinant drugs in process-friendly endotoxin-free bacterial factories targets to a lessened complexity of the purification process combined with minimized biological hazards during product ...

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