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Browsing by Subject "Sesquiterpenes"

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Biokatalytische Wege zur Darstellung funktioneller Sesquiterpene

Hartwig, Steffen (Hannover : Gottfried Wilhelm Leibniz Universität Hannover, 2016)

[no abstract]
Characterisation of a Recombinant Patchoulol Synthase Variant for Biocatalytic Production of Terpenes

Frister, Thore; Hartwig, Steffen; Alemdar, Semra; Schnatz, Katharina; Thöns, Laura; Scheper, Thomas; Beutel, Sascha (New York City, NY : Springer Humana Press Inc., 2015)

The patchoulol synthase (PTS) is a multi-product sesquiterpene synthases which is the central enzyme for biosynthesis of patchouli essential oil in the patchouli plant. Sesquiterpene synthases catalyse the formation of ...
Cyclopropylmethyldiphosphates are substrates for sesquiterpene synthases: experimental and theoretical results

Tran, Cong Duc; Dräger, Gerald; Struwe, Henry Frederik; Siedenberg, Lukas; Vasisth, Somi; Grunenberg, Jörg; Kirschning, Andreas (Cambridge : Royal Society of Chemistry, 2022)

New homo-sesquiterpenes are accessible after conversion of presilphiperfolan-8β-ol synthase (BcBOT2) with cyclopropylmethyl analogs of farnesyl diphosphate, and this biotransformation is dependent on subtle structural ...
Herstellung, Charakterisierung und Anwendung einer rekombinanten Patchoulolsynthese zur biokatalytischen Herstellung von Sesquiterpenen

Frister, Thore Hermann Wilhelm (Hannover : Gottfried Wilhelm Leibniz Universität Hannover, 2015)

[no abstract]
Heterologous Expression, Purification, and Biochemical Characterization of α-Humulene Synthase from Zingiber zerumbet Smith

Alemdar, Semra; Hartwig, Steffen; Frister, Thore; König, Jan Christoph; Scheper, Thomas; Beutel, Sascha (New York City, NY : Springer Humana Press Inc., 2016)

The α-humulene synthase from Zingiber zerumbet Smith was expressed as a polyhistidine-tagged protein in an E. coli BL21(DE3) strain. Induction time and inductor (isopropyl-β-D-thiogalactopyranoside) concentration were ...
Improved production and in situ recovery of sesquiterpene (+)-zizaene from metabolically-engineered E. Coli

Aguilar Cascante, Francisco José; Scheper, Thomas; Beutel, Sascha (Basel : MDPI AG, 2019)

The sesquiterpene (+)-zizaene is the direct precursor of khusimol, the main fragrant compound of the vetiver essential oil from Chrysopogon zizanioides and used in nearly 20% of men’s fine perfumery. The biotechnological ...
Oxygenated bisabolane fucosides from Carthamus lanatus L.

Mikhova, Bozhanka; Duddeck, Helmut; Taskova, Rilka M.; Mitova, Maya; Alipieva, Kalina (Berlin : De Gruyter, 2004)

The aerial parts of Carthamus lanatus (Asteraceae) afforded four new oxygenated bisabolane fucosides, 10-hydroperoxy-bisabola-2,11-diene 7-O-β-D-fucopyranoside, 11-hydroperoxy-bisabola-2,9-diene 7-O-β-D-fucopyranoside, ...
Sesquiterpene Backbones Generated by Sesquiterpene Cyclases: Formation of iso-Caryolan-1-ol and an Isoclovane

Struwe, Henry; Schrödter, Finn; Spinck, Hanke; Kirschning, Andreas (Washington, DC : ACS, 2023)

New sesquiterpene backbones are accessible after incubation of caryolan-synthase (GcoA) and presilphiperfolan-8-β-ol synthase (BcBOT2) with a non-natural farnesyldiphosphate in which the central olefinic double bond is ...
SUMO-fusion, purification, and characterization of a (+)-zizaene synthase from Chrysopogon zizanioides

Hartwig, Steffen; Frister, Thore; Alemdar, Semra; Li, Zhaopeng; Scheper, Thomas; Beutel, Sascha (Cambridge, MA : Elsevier Academic Press, 2015)

An uncharacterized plant cDNA coding for a polypeptide presumably having sesquiterpene synthase activity, was expressed in soluble and active form. Two expression strategies were evaluated in Escherichia coli. The enzyme ...
Whole-Cell Biocatalytical Production of the Sesquiterpene Presilphiperfolan-8β-ol in Pathway-Engineered Escherichia coli

Wildhagen, Maik; Pudenz, Tabea; Nguyen, Trang; Kirschning, Andreas; Beutel, Sascha (Weinheim : Wiley-VCH Verl., 2023)

Fermentative whole-cell production in Escherichia coli offers the option of producing complex natural products such as terpenes from simple, inexpensive, and sustainable carbon sources. The aim of this study was to develop ...

Now showing items 1-10 of 10

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