Browsing by Subject "Two-dimensional gel electrophoresis"

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Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures

Vanz, Ana Leticia; Nimtz, Manfred; Rinas, Ursula (London : BioMed Central Ltd., 2014)

Background: Pichia pastoris is a popular yeast preferably employed for secretory protein production. Secretion is not always efficient and endoplasmic retention of proteins with aberrant folding properties, or when produced ...
GelMap - A novel software tool for building and presenting proteome reference maps

Rode, Christina; Senkler, Michael; Klodmann, Jennifer; Winkelmann, Traud; Braun, Hans-Peter (New York, NY [u.a.] : Elsevier, 2011)

Protein separation by two-dimensional gel electrophoresis is of central importance for proteomics. Upon combination with systematic protein identifications by mass spectrometry, large data sets are routinely generated in ...
The metabolic potential of Escherichia coli BL21 in defined and rich medium

Li, Zhaopeng; Nimtz, Manfred; Rinas, Ursula (London : BioMed Central Ltd., 2014)

Background: The proteome reflects the available cellular machinery to deal with nutrients and environmental challenges. The most common E. coli strain BL21 growing in different, commonly employed media was evaluated using ...

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